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Dataset Identifier

Metadata
datasetIdentifierPASS00037
datasetTypeMSMS
submitterChristina Ludwig <ludwig@imsb.biol.ethz.ch>
submitter_organization
lab_head_full_name
lab_head_email
lab_head_organization
lab_head_country
datasetTagOliveira_p_yeast_met
datasetTitleRegulation of yeast central metabolism by enzyme phosphorylation
publicReleaseDate2012-06-01 00:00:00
finalizedDate
summaryAs a frequent post-translational modification, reversible protein phosphorylation regulates many cellular processes. Although several hundred phosphorylation sites have been mapped to metabolic enzymes in Saccharomyces cerevisiae, functionality was demonstrated for few of them. Here we identify enzyme phosphorylation events that are important in regulating the functioning of the network of 204 enzymes in yeast central carbon and amino acid metabolism. Specifically, we identified 35 differently phosphorylated enzymes during growth under five conditions, and combined these data with previously determined protein abundances and flux estimates. Correlation of intracellular fluxes with total or phosphoprotein abundances allowed us to assign function to eight previously unknown phosphorylation events, doubling the number of described functional phosphoenzymes in central carbon and amino acid metabolism. Finally, we validated the functional impact of selected enzyme phosphosites on the functioning of the entire metabolic network through genetic removal of phosphosites and by specific phosphosite quantification using targeted mass spectrometry.
contributorsAna Paula Oliveira
Christina Ludwig
Paola Picotti
Ruedi Aebersold
Uwe Sauer
publicationunpublished so far
growth
treatment
extraction
separation
digestion
acquisition
informatics
instrumentsThermo Scientific LTQ-FT-ICR
Thermo Scientific LTQ Orbitrap
speciesSaccharomyces cerevisiae
massModificationsstatic: C+57.021464, variable: S+79.966331, variable: T+79.966331, variable: Y+79.966331,

Official URL for this dataset: http://www.peptideatlas.org/PASS/PASS00037
To access files via FTP, use credentials:
Servername: ftp.peptideatlas.org
Username: PASS00037
Password: VJ4864o

Or use your browser's FTP mode: ftp://PASS00037:VJ4864o@ftp.peptideatlas.org/


Listing of files:

 1.3G Jan 31  2012 20081223_27_pp_Glu.mzXML
 1.3G Jan 31  2012 20081223_28_pp_Glu.mzXML
 1.3G Jan 31  2012 20081223_29_pp_Glu.mzXML
 1.2G Jan 31  2012 20081223_31_pp_Eth.mzXML
 1.3G Jan 31  2012 20081223_32_pp_Eth.mzXML
 1.3G Jan 31  2012 20081223_33_pp_Eth.mzXML
 1.2G Jan 31  2012 20081223_35_pp_Gal.mzXML
 1.3G Jan 31  2012 20081223_36_pp_Gal.mzXML
 1.4G Jan 31  2012 20081223_37_pp_Gal.mzXML
 1.4G Jan 31  2012 20081223_39_pp_CX.mzXML
 1.4G Jan 31  2012 20081223_40_pp_CX.mzXML
 1.4G Jan 31  2012 20081223_41_pp_CX.mzXML
 1.4G Jan 31  2012 20081223_43_pp_An.mzXML
 1.4G Jan 31  2012 20081223_44_pp_An.mzXML
 1.4G Jan 31  2012 20081223_45_pp_An.mzXML
 973M Jan 31  2012 B08-11178_Phospho_Glu_R1_p.mzXML
 970M Jan 31  2012 B08-11179_Phospho_Glu_R2_p.mzXML
 960M Jan 31  2012 B08-11180_Phospho_Glu_R3_p.mzXML
 979M Jan 31  2012 B08-11186_Phospho_Eth_R1_p.mzXML
 962M Jan 31  2012 B08-11187_Phospho_Eth_R2_p.mzXML
 984M Jan 31  2012 B08-11188_Phospho_Eth_R3_p.mzXML
 909M Jan 31  2012 B08-11190_Phospho_CX_R1_p.mzXML
 934M Jan 31  2012 B08-11191_Phospho_CX_R2_p.mzXML
 1.1G Jan 31  2012 B08-11194_Phospho_Ana_R1_p.mzXML
 1.1G Jan 31  2012 B08-11195_Phospho_Ana_R2_p.mzXML
 1.1G Jan 31  2012 B08-11196_Phospho_Ana_R3_p.mzXML
  47K Jan 31  2012 Overview_files_shotgun_yeast_metabolism_phospho.xlsx
 1.7K Jan 31  2012 PASS00037_DESCRIPTION.txt

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