Metadata |
datasetIdentifier | PASS00037 |
datasetType | MSMS |
submitter | Christina Ludwig <ludwig@imsb.biol.ethz.ch> |
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datasetTag | Oliveira_p_yeast_met |
datasetTitle | Regulation of yeast central metabolism by enzyme phosphorylation |
publicReleaseDate | 2012-06-01 00:00:00 |
finalizedDate | |
summary | As a frequent post-translational modification, reversible protein phosphorylation regulates many cellular processes. Although several hundred phosphorylation sites have been mapped to metabolic enzymes in Saccharomyces cerevisiae, functionality was demonstrated for few of them. Here we identify enzyme phosphorylation events that are important in regulating the functioning of the network of 204 enzymes in yeast central carbon and amino acid metabolism. Specifically, we identified 35 differently phosphorylated enzymes during growth under five conditions, and combined these data with previously determined protein abundances and flux estimates. Correlation of intracellular fluxes with total or phosphoprotein abundances allowed us to assign function to eight previously unknown phosphorylation events, doubling the number of described functional phosphoenzymes in central carbon and amino acid metabolism. Finally, we validated the functional impact of selected enzyme phosphosites on the functioning of the entire metabolic network through genetic removal of phosphosites and by specific phosphosite quantification using targeted mass spectrometry. |
contributors | Ana Paula Oliveira
Christina Ludwig
Paola Picotti
Ruedi Aebersold
Uwe Sauer |
publication | unpublished so far |
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digestion | |
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informatics | |
instruments | Thermo Scientific LTQ-FT-ICR
Thermo Scientific LTQ Orbitrap |
species | Saccharomyces cerevisiae |
massModifications | static: C+57.021464, variable: S+79.966331, variable: T+79.966331, variable: Y+79.966331, |