| Metadata |
| datasetIdentifier | PASS00081 |
| datasetType | Other |
| submitter | Vincent Geoghegan <vincentgeoghegan@hotmail.com> |
| submitter_organization | |
| lab_head_full_name | |
| lab_head_email | |
| lab_head_organization | |
| lab_head_country | |
| datasetTag | methylSILACMethArg |
| datasetTitle | methyl SILAC labelled Jurkat T-lymphocytes or primary human T-lymphocytes |
| publicReleaseDate | 2012-08-04 00:00:00 |
| finalizedDate | 2012-07-30 03:06:53 |
| summary | The aim of this project was to identify arginine methylation sites. Jurkat T-lymphocytes or primary human T-lymphocytes were methylSILAC labelled to provide increased confidence in identification of methylation sites. Peptides were separated by HILIC, SCX or IEF |
| contributors | Vincent Geoghegan, Thomas Uhlmann, Benjamin Thomas, David Trudgian, Gabriela Ridlova, Oreste Acuto |
| publication | Uhlmann,T, Geoghegan, V, Thomas, B, Ridlova, G, Trudgian, D and Oreste Acuto
A method for large-scale identification of protein
arginine methylation
Molecular and Cellular Proteomics
Submitted |
| growth | Growth in RPMI |
| treatment | Urea lysis, digest with Chymotrypsin or trypsin |
| extraction | acetone precipitation |
| separation | HILIC, SCX, IEF |
| digestion | Trypsin or Chymotrypsin |
| acquisition | See paper |
| informatics | See paper |
| instruments | Thermo Scientific LTQ Orbitrap, Qexactive |
| species | Human |
| massModifications | static C+57.021464 methylSILAC labelled |
