≡   PeptideAtlas Links Seattle Proteome Center

PeptideAtlas: Home Overview Contacts Publications Software Database Schema Feedback Funding FAQ
Atlas Data: Data Repository HPPP Data Central PeptideAtlas Builds PeptideAtlas Exports THISP Search Database
Contribute Data

Related: SRMAtlas PASSEL SWATHAtlas
Spectral Libs: Libraries + Info SpectraST Search

Glossary/Terms: Atlas nomenclature Protein ID terms

LOG IN
MENU
Log In
Search All Builds Select Build Stats & Lists Peps & Prots for Default Builds Summarize Peptide View Ortholog Group Current Build Peptide Protein Queries Browse Peptides Browse Proteins Browse Core Proteome Browse PTM Summary Browse Protein By Experiment Compare Proteins in 2 Builds Search Proteins Pathways HPP Protein Lists SRMAtlas Query Transitions Transition Lists SRMAtlas Builds PASSEL Experiments PASSEL Data Submission SWATH/DIA Download Library Custom Library Assess Library Upload Library

View Dataset


Email address      Password

Dataset Identifier

Metadata
datasetIdentifierPASS00131
datasetTypeSRM
submitterPaola Picotti <paola.picotti@bc.biol.ethz.ch>
submitter_organization
lab_head_full_namePaola Picotti
lab_head_emailpaola.picotti@bc.biol.ethz.ch
lab_head_organizationETH
lab_head_countrySwitzerland
datasetTagDiaShift
datasetTitleYeast Diauxic Shift Dataset
publicReleaseDate2013-04-02 00:00:00
finalizedDate
summaryThe diauxic shift in Saccharomyces cerevisiae is an ideal model to study how eukaryotic cells re-adjust their metabolism from glycolytic to gluconeogenic operation. In this work, we generated time-resolved physiological data, quantitative metabolome (69 intracellular metabolites) and proteome (72 enzymes) profiles. We found that the diauxic shift is accomplished by three key events that are temporally organized: (i) a reduction of the glycolytic flux and the production of storage compounds before glucose depletion, mediated by down-regulation of phosphofructokinase and pyruvate kinase reactions; (ii) upon glucose exhaustion, the reversion of carbon flow through glycolysis and onset of the glyoxylate cycle operation triggered by an increased expression of the enzymes that catalyze the malate synthase and cytosolic citrate synthase reactions; and (iii) in the later stages of the adaptation, the shutting down of the pentose phosphate pathway with a change in NADPH regeneration. Moreover, we identified the transcription factors associated with the observed changes in protein abundances. Taken together, our results represent an important contribution towards a systems-level understanding of how this adaptation is realized.
contributorsZampar, G.G., Kümmel, A.2, Ewald, J., Jol, S., Niebel, B., Picotti, P., Aebersold, R., Sauer, U., Zamboni, N., and Heinemann, M.
publicationZampar, GG, Kümmel, A, Ewald, J, Jol, S, Niebel, B, Picotti, P, Aebersold, R, Sauer, U, Zamboni, N, and Heinemann, M., Temporal system-level organization of the switch from glycolytic to gluconeogenic operation in yeast, Mol Sys Biol, submitted
growthsee manuscript
treatmentsee manuscript
extractionsee manuscript
separationsee manuscript
digestionsee manuscript
acquisitionSRM acquisition, see manuscript
informaticssee manuscript
instrumentsABSciex, 4000QTRAP
speciesS. cerevisiae
massModificationsC+57.021464
15N labelled AA

Official URL for this dataset: http://www.peptideatlas.org/PASS/PASS00131
To access files via FTP, use credentials:
Servername: ftp.peptideatlas.org
Username: PASS00131
Password: QF2869zq

Or use your browser's FTP mode: ftp://PASS00131:QF2869zq@ftp.peptideatlas.org/


Listing of files:

  25M Dec  3  2012 Diauxic_TimeCourse_Final_21May08.wiff
  14M Dec  3  2012 Diauxic_TimeCourse_Final_21May08.wiff.scan
 105K Dec  3  2012 MH_DiauxicShift_TransitionList.tsv.csv
 2.1K Dec  3  2012 PASS00131_DESCRIPTION.txt

Browse FTP upload area