| Metadata |
| datasetIdentifier | PASS00442 |
| datasetType | MSMS |
| submitter | David Trudgian <david.trudgian@utsouthwestern.edu> |
| submitter_organization | University of Texas Southwestern Medical Center |
| lab_head_full_name | Kim Orth |
| lab_head_email | Kim.Orth@UTSouthwestern.edu |
| lab_head_organization | University of Texas Southwestern Medical Center |
| lab_head_country | USA |
| datasetTag | T6SS_Effectors |
| datasetTitle | New domain reveals functionally diverse Type VI Secretion System effectors |
| publicReleaseDate | 2014-05-22 00:00:00 |
| finalizedDate | 2014-05-22 10:54:15 |
| summary | Vibrio parahaemolyticus cells were grown in minimal media (M9 + 3% NaCl) with addition of 5 uM phenamil (to simulate growth on surface) for 5 hours in triplicates, and secreted proteins were precipitated from the media, ran on a gel (10 mm) and excised. Two strains were used:
R = POR1 delta:opaR (T6SS1 +)
H = POR1 delta:opaR delta:hcp1 (T6SS1 -)
Triplicate samples were analyzed for R and H.
The goal of this experiment is to identify proteins that are secreted from the T6SS1+ strain (R) and not from the T6SS- strain (h). These are potentially effector proteins that are secreted via the type VI secretion system 1 (T6SS1). |
| contributors | Dor Salomon
Lisa N. Kinch
David C. Trudgian
Xiaofeng Guo
Nick V. Grishin
Hamid Mirzaei
Kim Orth |
| publication | Dor Salomon, Lisa N. Kinch, David C. Trudgian, Xiaofeng Guo, John A. Klimko, Nick V. Grishin, Hamid Mirzaei, and Kim Orth, Marker for type VI secretion system effectors, PNAS. |
| growth | |
| treatment | |
| extraction | |
| separation | |
| digestion | |
| acquisition | |
| informatics | |
| instruments | Thermo Scientific Q Exactive |
| species | Vibrio parahaemolyticus |
| massModifications | static: C+57.021464 |
