| Metadata |
| datasetIdentifier | PASS00454 |
| datasetType | SRM |
| submitter | Anne Gonzalez de Peredo <gonzalez@ipbs.fr> |
| submitter_organization | CNRS |
| lab_head_full_name | B. Monsarrat |
| lab_head_email | monsarrat@ipbs.fr |
| lab_head_organization | CNRS |
| lab_head_country | France |
| datasetTag | UPJ_urine |
| datasetTitle | clinical validation of potential urinary biomarkers of ureteropelvic junction (UPJ) obstruction in newborns |
| publicReleaseDate | 2014-05-01 00:00:00 |
| finalizedDate | 2014-03-26 07:27:09 |
| summary | clinical validation of 7 potential biomarkers of ureteropelvic junction (UPJ) obstruction in urine samples from newborns (n=31) |
| contributors | Lacroix, C.
Caubet, C.
Gonzalez de Peredo, A.
Stella, A.
Garrigues, L.
Breuil, B.
Decramer Stéphane
Burlet-Schiltz Odile
Monsarrat Bernard
Schanstra Joost-Peter
Bascands Jean-Loup |
| publication | Lacroix C, Caubet C, Gonzalez-de-Peredo A, Breuil B, Raevel A, Bouyssié D, Le Gall C, Massoubre A3, Klein J, Decramer S, Sabourdy F, Bandin F, Burlet-Schiltz O, Monsarrat B, Schanstra J-P, Bascands J-L, Label-free quantitative urinary proteomics identifies the arginase pathway as a new player in congenital obstructive nephropathy, Mol Cell Proteomics, submitted |
| growth | |
| treatment | 3 patient groups: healthy (n=10), mild (n=13), severe (n=8) |
| extraction | urine samples were desalted and lyophilized, proteins were redissolved in Laemli buffer and concentrated on SDS-PAGE (1 gel band) for in-gel digestion |
| separation | |
| digestion | Trypsin in-gel digestion of the total urinary protein mixture |
| acquisition | MRM acquisition on AB 5500 Q-trap. Monitoring of 24 targeted peptides (corresponding to 7 urinary candidate proteins plus 2 yeast proteins used as internal standards), 25 targeted peptide ions. The corresponding heavy forms (either AQUA or Pepotec synthetic peptides) of the 25 targeted peptide ions were also monitored. Three transitions were monitored for each of the 50 peptide ion forms (total: 150 transitions). |
| informatics | Skyline data processing + QUASAR, AuDIT |
| instruments | AB Sciex QTRAP 5500 |
| species | Human |
| massModifications | C+57.021464 |
