Metadata |
datasetIdentifier | PASS00512 |
datasetType | MSMS |
submitter | Michael Hoopmann <Michael.Hoopmann@systemsbiology.org> |
submitter_organization | Institute for Systems Biology |
lab_head_full_name | Robert Moritz |
lab_head_email | robert.moritz@systemsbiology.org |
lab_head_organization | Institute for Systems Biology |
lab_head_country | United States |
datasetTag | Sludge041010 |
datasetTitle | Sludge from a sewage treatment facility in Luxembourg, collected on 04 Oct 2010. Samples were separated on a gel, cut into fractions, then digested to peptides. |
publicReleaseDate | 2015-06-17 00:00:00 |
finalizedDate | 2015-06-17 11:14:35 |
summary | Sludge from a sewage treatment facility in Luxembourg, collected on 04 Oct 2010. Samples were separated on a gel, cut into fractions, then digested to peptides. Data were searched using a bacterial metaproteome representing the mixed species of bacteria observed at that time point in the dynamic population. A comparative analysis was performed using sludge collected on 25 Jan 2011 from the same site. The comparative search results for the 25 Jan 2011 dataset are found here. Raw mass spectra for 25 Jan 2011 sludge are found in PASS00359. |
contributors | Hugo Roume, Anna Heintz-Buschart, Emilie EL Muller, Patrick May, Venkata Satagopam, Cédric Laczny, Shaman Narayanasamy, Laura A. Lebruna, Michael R. Hoopmann, James M Schupp, Thomas Sauter, Paul S Keim, Robert L. Moritz, Paul Wilmes |
publication | Roume H, Heintz-Buschart A, Muller EEL, May P, Satagopam VP, Laczny CC, et al. Comparative integrated omics: identification of key functionalities in microbial community-wide metabolic networks. Npj Biofilms And Microbiomes. 2015;1. doi: 10.1038/npjbiofilms.2015.7 http://www.nature.com/articles/npjbiofilms20157 |
growth | |
treatment | |
extraction | |
separation | Separated by SDS-PAGE and cut into 1 mm bands |
digestion | In-gel tryptic digest |
acquisition | 96 fractions were pooled in pairs (48 total samples) and analyzed by 1 hr LC/MS using shotgun analysis and HCD fragmentation. |
informatics | Converted to mzXML and analyzed using TPP (X!Tandem, Peptide Prophet, Protein Prophet, iProphet). |
instruments | Thermo Scientific Orbitrap Elite |
species | Multi-species bacterial sludge |
massModifications | C+57.021464
M+15.994915 |