Metadata |
datasetIdentifier | PASS00589 |
datasetType | MSMS |
submitter | Roland Bruderer <bruderer@biognosys.ch> |
submitter_organization | BiognoSYS AG and ETH Zrurich |
lab_head_full_name | Lukas Reiter |
lab_head_email | reiter@biognosys.ch |
lab_head_organization | Research and Development |
lab_head_country | Switzerland |
datasetTag | HRMProfilingComp |
datasetTitle | Extending the limits of quantitative proteome profiling with data-independent acquisition and application to acetaminophen treated 3D liver microtissues |
publicReleaseDate | 2015-03-01 00:00:00 |
finalizedDate | 2015-08-27 02:44:45 |
summary | The data set repersents a fair comparison of shotgun proteomics with HRM (swath type DIA). Additionally, a human liver microtissue was profiled with HRM. |
contributors | Roland Bruderer, Oliver M. Bernhardt, Tejas Gandhi, Saša M. Miladinović, Lin-Yang Cheng, Simon Messner, Tobias Ehrenberger, Vito Zanotelli, Yulia Butscheid, Claudia Escher, Olga Vitek, Oliver Rinner & Lukas Reiter |
publication | 25724911 |
growth | The microtissues were produced and cultured as decribed in the publication. |
treatment | The microtissues were treated with acetaminophen as describe in the publication. |
extraction | |
separation | |
digestion | The samples were reduced by DTT, alkylated with iodoacetamin and digested in solution by trpysin. |
acquisition | The samples were acquired as described in the publication. |
informatics | The data was analyzed using Maxquant 1.4.1.2 and Specrtonaut 5.0. |
instruments | Thermo Scientific Q Exactive |
species | Human |
massModifications | static: C+57.021464, variable: K+42.010565, M+15.994915 |