| Metadata |
| datasetIdentifier | PASS00939 |
| datasetType | SRM |
| submitter | Linda Vuorijoki <lkvuor@utu.fi> |
| submitter_organization | University of Turku |
| lab_head_full_name | Eva-Mari Aro |
| lab_head_email | evaaro@utu.fi |
| lab_head_organization | University of Turku |
| lab_head_country | Finland |
| datasetTag | IsaR1_SRM |
| datasetTitle | Acclimation of oxygenic photosynthesis to iron starvation is controlled by the sRNA IsaR1 in cyanobacteria |
| publicReleaseDate | 2017-03-03 00:00:00 |
| finalizedDate | 2016-10-18 03:28:09 |
| summary | Oxygenic photosynthesis crucially depends on proteins that possess Fe2+ or Fe/S complexes as co-factors or prosthetic groups. Here we show that the regulatory sRNA IsaR1 (Iron-stress activated RNA 1) plays a pivotal role in acclimation to low iron conditions. The IsaR1 regulon consists of more than 15 direct targets, including Fe2+-containing proteins involved in photosynthetic electron transfer, the detoxification of anion radicals, citrate cycle, and in tetrapyrrole biogenesis. IsaR1 is essential to maintain physiological levels of Fe/S cluster biogenesis proteins during iron deprivation. Consequently, IsaR1 affects the acclimation of the photosynthetic apparatus to iron starvation at three levels: (i) directly, via posttranscriptional repression of gene expression and indirectly, (ii) via the suppression of pigment biosynthesis and (iii) Fe/S cluster co-factor. Homologs of IsaR1 are widely conserved throughout the cyanobacterial phylum. We conclude that IsaR1 is a riboregulator of central importance. These findings provide a new perspective for studies of the regulation of iron homeostasis in photosynthetic organisms. |
| contributors | Jens Georg, Gergana Kostova, Linda Vuorijoki, Verena Schön, Taro Kadowaki, Tuomas Huokko, Yagut Allahverdiyeva-Rinne, Yukako Hihara, Matthias E. Futschik, Eva-Mari Aro, Wolfgang R. Hess |
| publication | Georg, J. et al., Acclimation of oxygenic photosynthesis to iron starvation is controlled by the sRNA IsaR1 in cyanobacteria, Cell, submitted |
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| informatics | |
| instruments | Thermo Scientific TSQ Vantage |
| species | Synechocystis sp. PCC 6803 |
| massModifications | Static: C+57.021464 |
