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Dataset Identifier

Metadata
datasetIdentifierPASS01115
datasetTypeSRM
submitterVictor Faundez <vfaunde@emory.edu>
submitter_organizationEmory University
lab_head_full_nameVictor Faundez
lab_head_emailvfaunde@emory.edu
lab_head_organizationEmory University
lab_head_countryUnited States
datasetTagMSB-3677-3720-3747
datasetTitleGenealogical Proteomics Menkes Disease
publicReleaseDate2018-01-01 00:00:00
finalizedDate
summaryTriple SILAC labeling of human fibroblasts from families affected by Menkes disease
contributorsVictor Faundez
publicationRare Disease Mechanisms Identified by Genealogical Proteomics of Copper Homeostasis Mutant Pedigrees. Zlatic et. al. Submitted
growthSeven passages in SILAC media
treatment
extractionDetergent Cell Extract
separationGel digests were analyzed by nano LC/MS/MS with a Waters NanoAcquity HPLC system
digestionGel pieces were processed using a robot (ProGest, DigiLab) with the following protocol:

· Washed with 25mM ammonium bicarbonate followed by acetonitrile.

· Reduced with 10mM dithiothreitol at 60°C followed by alkylation with 50mM iodoacetamide at RT.

· Digested with trypsin (Promega) at 37°C for 4h.

· Quenched with formic acid and the supernatant was analyzed directly without further processing.
acquisitiondata-dependent mode, with MS and MS/MS performed in the Orbitrap at

70,000 FWHM resolution and 17,500 FWHM resolution, respectively. The fifteen most abundant ions were

selected for MS/MS.
informaticsMaxQuant software 1.4.1.2
instrumentsThermoFisher Q Exactive
speciesHuman
massModificationsOxidation (M), Acetyl (Protein N-term), 13C6/15N2 (K), 13C6/15N4 (R), 4H2 (K), 13C6 (R)

Official URL for this dataset: http://www.peptideatlas.org/PASS/PASS01115
To access files via FTP, use credentials:
Servername: ftp.peptideatlas.org
Username: PASS01115
Password: AT7444k

Or use your browser's FTP mode: ftp://PASS01115:AT7444k@ftp.peptideatlas.org/


Listing of files:

 4.0K Oct 20  2017 MSB-3677
 4.0K Oct 20  2017 MSB-3720
 4.0K Oct 20  2017 MSB-3747
 1.4K Oct 20  2017 PASS01115_DESCRIPTION-2017-09-20_064555.txt
 1.4K Oct 20  2017 PASS01115_DESCRIPTION.txt

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