| Metadata |
| datasetIdentifier | PASS01468 |
| datasetType | SWATH |
| submitter | Valentine Cyriaque <valentine.cyriaque@umons.ac.be> |
| submitter_organization | UMONS, Mons, Belgium |
| lab_head_full_name | Proteomics and microbiology |
| lab_head_email | ruddy.wattiez@umons.ac.be |
| lab_head_organization | UMONS |
| lab_head_country | Belgium |
| datasetTag | mating_cocultVC |
| datasetTitle | Functional profile of conjugation-mating co-cultures at increasing lead concentrations |
| publicReleaseDate | 2019-10-20 00:00:00 |
| finalizedDate | 2019-10-16 08:34:43 |
| summary | Functiona; profile of cbi-membered co-culture gathering a plasmid (pKJK5, IncP) donor and a plamid recipient |
| contributors | Valentine Cyriaque
Ruddy Wattiez |
| publication | unpublished yet |
| growth | Co-culture (4 days) in 3-time diluted LB at 16C-120 RPM
of plasmid donor Pseudomonas putida KT2440plpp ;Cherry KmR carrying either pKJK5-TcR plasmid or pKJK5-TcR-pbrTRABCD (starting 6,000 cells)
and plasmid recipient (60,000 starting cells) either Variovorax paradoxus B4 or Delftia acidovorans SPH-1 |
| treatment | |
| extraction | PreOmics Ist 8x kit (Martinsried, Germany ) |
| separation | |
| digestion | PreOmics Ist 8x kit (Martinsried, Germany ) |
| acquisition | |
| informatics | Library Processing_Protein Pilot
SWATH processing PeakView 2.2 & Markerview 1.3.1 |
| instruments | SCIEX Triple Tof 6600 |
| species | Pseudomonas putida KT2440
Variovorax paradoxus B4
Delftia acidovorans SPH-1 |
| massModifications | none |
