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Spectrum Library Central at PeptideAtlas

Welcome to the Spectrum Library Central, hosted at PeptideAtlas. We provide a list and links to Spectrum Libraries built specifically for spectrum library searching of tandem mass-spec data.

Spectral Library Search Projects

Spectral library searching, unlike sequence database searches, involve finding the best match of an acquired MS/MS spectrum to a library of pre-searched spectra for which the sequences have been determined. This approach can be hundreds of times faster than traditional searching, with comparable or better accuracy.

SpectraST Developed at ISB by H.Lam. Included as part of the Trans-Proteomic Pipeline (TPP) distribution. A stand-alone Windows version can now be downloaded from the ISB download section below. An online query form is also available. For more information, please consult the SpectraST information page
NIST MS Search The National Institute of Standards and Technology (NIST) distributes an updated version of their NIST MS Search Program for Windows, which includes the ability to perform Peptide searches via spectrum matching. The NIST Peptide Library Search Engine is also available from the NIST download section below.
X! Hunter Developed by the The Global Proteome Machine Organization. Online query forms for submitting a file or a single spectrum are available.
BlibSearch Part of the BiblioSpec suite, developed at the MacCoss Lab at the University of Washington.
Bonanza A spectral comparison algorithm developed by the Andrews Lab at the University of Michigan. (No website or data yet).

Download Spectrum Libraries

Links to download reference spectra and related documentation from various sources.

   ISB

Libraries built from PeptideAtlas data by H. Lam, specifically for use with SpectraST.

File Size Date md5 checksum* Download
ISB_Hs_plasma_20070706_PUBLIC.zip 218MB 2007-07-20 
d81cf3d9e5e1966541ed801d61b42de7
Consensus spectral libraries constructed from the 40 public datasets in Human Plasma PeptideAtlas (Human)
ISB_Dm-phospho_20080313.zip 161MB 2008-03-13 
6cee55224e2cbfb7dfe64d47800788b0
Quality-filtered consensus spectral libraries of Drosophila melanogaster peptides enriched for phosphorylation by 3 different methods. (Bodenmiller et al. (2007) Mol. Systems Biol. 3, 139 and (2008) Nature Biotechnology 26, 1339-1340 )
ISB_Ce-phospho_20080313.zip 39MB 2008-03-13 
5e01bbb1a22d91ea34720fe4db00b8d1
Quality-filtered consensus spectral libraries of Caenorhabditis elegans peptides enriched for phosphorylation. (Bodenmiller et al. (2008) Nature Biotechnology 26, 1339-1340 )
ISB_Hs-phospho_20080428.zip 21MB 2008-04-28 
036e4907522c47775c55c204f3c47c56
Quality-filtered consensus spectral libraries of Homo sapiens peptides enriched for phosphorylation. (Bodenmiller et al. (2008) Nature Biotechnology 26, 1339-1340)
I recognize that this data is the property of Cell Signaling Technology and agree not to use it for any commercial purpose whatsoever without their express written consent.
ISB_Sc-phospho_20080313.zip 94MB 2008-03-13 
d00aa125f77f95f9911c1c365f9ddd0f
Quality-filtered consensus spectral libraries of Saccharomyces cerevisiae peptides enriched for phosphorylation. (Bodenmiller et al. (2008) Nature Biotechnology 26, 1339-1340 )
SpectraST_2008_07_01.zip 27MB 2008-07-01 
545600349c50613c03c3c5a65974ffec
Stand-alone Windows native version of SpectraST (also available as part of the TPP distribution)


   NIST

These libraries are built by S. Stein at NIST; more information and related software can be found at peptide.nist.gov. The Institute for Systems Biology asks that anyone wishing to download any of the NIST Libraries of Peptide Fragmentation Spectra read the following paragraph and acknowledge before downloading:

"I recognize that this data collection, the NIST Library of Peptide Fragmentation Spectra, is copyrighted by the Department of Commerce, U.S. Government and shall not be redistributed in any manner without a valid Distribution Agreement. To receive such an agreement, contact the Standard Reference Data Program, National Institute of Standards and Technology, at data*at*nist.gov or 301-975-2008."   

File Size Date md5 checksum* Download
NIST_aurum_TOFTOF_v3.0_2008-07-15.msp.zip 508K 2009-05-29 
ab03a7c84eb19615e75d2346061bfe49
NIST Library of Peptide Ion Fragmentation Spectra (Aurum Dataset of purchased proteins)
NIST_aurum_TOFTOF_v3.0_2008-07-15.nist.zip 1.2M 2009-05-29 
10a97de56f17431c7525c2e2689b428b
NIST Library of Peptide Ion Fragmentation Spectra (Aurum Dataset) -- NIST format
NIST_aurum_TOFTOF_v3.0_2008-07-15_7AA.splib.zip 1.1M 2009-05-29 
4e724b743219531036d4878e08591c10
NIST Library of Peptide Ion Fragmentation Spectra (Aurum Dataset) -- SpectraST format with short peptides (<7AA) excluded
NIST_bsa_IT_v3.0_2009-05-06.msp.zip 1.6M 2009-05-29 
dc92b5aa378c7eee2d57e93a22702e97
NIST Library of Peptide Ion Fragmentation Spectra (bsa)
NIST_bsa_IT_v3.0_2009-05-06.nist.zip 3.2M 2009-05-29 
86115b19767f2b1cc9be368d9f43d4a6
NIST Library of Peptide Ion Fragmentation Spectra (bsa) -- NIST format
NIST_bsa_IT_v3.0_2009-05-06.splib.zip 3.8M 2009-05-29 
16fc779ca6179048ef67b39ca1ec29b0
NIST Library of Peptide Ion Fragmentation Spectra (bsa) -- SpectraST format
NIST_c_elegans_IT_2009-10-28.msp.zip 174M 2010-06-30 
f8e8b5d9a03e990b099842564f71888a
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: C. elegans)
NIST_c_elegans_IT_2009-10-28.nist.zip 346M 2010-06-30 
eac113c7e4814985267b4c1429f8ad2e
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: C. elegans) -- NIST format
NIST_c_elegans_IT_2009-10-28_7AA.splib.zip 411M 2010-06-30 
754016ffa5dc3fceae7aa7ca2e1482de
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: C. elegans) -- SpectraST format with short peptides (<7AA) excluded
NIST_chicken_IT_v3.0_2009-04-15.msp.zip 7.9M 2009-05-29 
f44fb2da409780190e26c0827b1e1c9a
NIST Library of Peptide Ion Fragmentation Spectra (Chicken)
NIST_chicken_IT_v3.0_2009-04-15.nist.zip 15M 2009-05-29 
d58f2b09e8180ab0c56f4010edbc3667
NIST Library of Peptide Ion Fragmentation Spectra (Chicken) -- NIST format
NIST_chicken_IT_v3.0_2009-04-15_7AA.splib.zip 19M 2009-05-29 
5c1656e7d10f74638c26748b7a595c33
NIST Library of Peptide Ion Fragmentation Spectra (Chicken) -- SpectraST format with short peptides (<7AA) excluded
NIST_dradiodurans_IT_2010-03-30.msp.zip 15M 2010-06-30 
339b3601c5d720f2c21ca8f668858733
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: D. radiodurans)
NIST_dradiodurans_IT_2010-03-30.nist.zip 32M 2010-06-30 
4c548e8f801f7cc858a1599507686d1b
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: D. radiodurans) -- NIST format
NIST_dradiodurans_IT_2010-03-30_7AA.splib.zip 36M 2010-06-30 
7536b19bde6c42f194883bc7dfb2c173
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: D. radiodurans) -- SpectraST format with short peptides (<7AA) excluded
NIST_drosophila_IT_2010-03-25.msp.zip 193M 2010-06-30 
6f531f6136cea12c4f7dea7bf710ddf9
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Drosophila)
NIST_drosophila_IT_2010-03-25.nist.zip 379M 2010-06-30 
6d393c278488b924c6add89f765a8471
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Drosophila) -- NIST format
NIST_drosophila_IT_2010-03-25_7AA.splib.zip 458M 2010-06-30 
fc66a87ef711f6b94c4448aada254ec7
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Drosophila) -- SpectraST format with short peptides (<7AA) excluded
NIST_e_coli_IT_2009-11-25.msp.zip 88M 2010-06-30 
53ecf42d36a1142979c01f8df090faae
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: E. coli)
NIST_e_coli_IT_2009-11-25.nist.zip 173M 2010-06-30 
029bd56a085c3b3b976763e94ca23aaa
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: E. coli) -- NIST format
NIST_e_coli_IT_2009-11-25_7AA.splib.zip 206M 2010-06-30 
3cc59bb3160625147f2ee1ce9a07b0c2
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: E. coli) -- SpectraST format with short peptides (<7AA) excluded
NIST_hsa_IT_2009-08-03.msp.zip 3.9M 2010-06-30 
d6c8b4f614f8cd73016c6094a953d6c4
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Human Serum Albumin)
NIST_hsa_IT_2009-08-03.nist.zip 8.0M 2010-06-30 
b2d42a5b74f58c91292280bed5d8ea63
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Human Serum Albumin) -- NIST format
NIST_hsa_IT_2009-08-03_7AA.splib.zip 9.6M 2010-06-30 
9d3e5baf4dcb122cd500f4166ffc54c4
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Human Serum Albumin) -- SpectraST format with short peptides (<7AA) excluded
NIST_human_b2mg_IT_v3.0_2009-05-06.msp.zip 260K 2009-05-29 
b23ddec5023df08c9b074359497c4694
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Homo sapiens / Beta-2-microglobulin)
NIST_human_b2mg_IT_v3.0_2009-05-06.nist.zip 560K 2009-05-29 
aafe89afaced595a9701abd9b8cace34
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Homo sapiens / Beta-2-microglobulin) -- NIST format
NIST_human_b2mg_IT_v3.0_2009-05-06_7AA.splib.zip 624K 2009-05-29 
ba7e4f59afc5eec2a6b9ddd42bf8e4a2
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Homo sapiens / Beta-2-microglobulin) -- SpectraST format with short peptides (<7AA) excluded
NIST_human_crp_IT_v3.0_2009-05-06.msp.zip 108K 2009-05-29 
8e0c0ebdafa49f0637e23a670cb62dac
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Homo sapiens / C-reactive protein)
NIST_human_crp_IT_v3.0_2009-05-06.nist.zip 244K 2009-05-29 
9116eb32102f28138a83772a339200bc
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Homo sapiens / C-reactive protein) -- NIST format
NIST_human_crp_IT_v3.0_2009-05-06_7AA.splib.zip 232K 2009-05-29 
dfbda3bd315baef7aa8f60a1312bbe50
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Homo sapiens / C-reactive protein) -- SpectraST format with short peptides (<7AA) excluded
NIST_human_IT_2010-01-14.msp.zip 596M 2010-06-30 
9b9ea8e31582d5dc4413186332a1c2b8
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Homo sapiens)
NIST_human_IT_2010-01-14.nist.zip 1.1G 2010-06-30 
fb40e7eabd238b26f4a638e7a79160b4
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Homo sapiens) -- NIST format
NIST_human_IT_2010-01-14_7AA.splib.zip 1.4G 2010-06-30 
2ec31a41de56a98ca5248f252374c241
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Homo sapiens) -- SpectraST format with short peptides (<7AA) excluded
NIST_human_QTOF_2010-03-02.msp.zip 19M 2010-06-30 
69f9e7b4df80e4c64100063afc8b8c57
NIST Library of Peptide Ion Fragmentation Spectra (QTOF: Homo sapiens)
NIST_human_QTOF_2010-03-02.nist.zip 39M 2010-06-30 
7c1f68211f8a3c19e1b8a1fe30c45f64
NIST Library of Peptide Ion Fragmentation Spectra (QTOF: Homo sapiens) -- NIST format
NIST_human_QTOF_2010-03-02_7AA.splib.zip 46M 2010-06-30 
95d5472a7725673ddef7e3485aced55b
NIST Library of Peptide Ion Fragmentation Spectra (QTOF: Homo sapiens) -- SpectraST format with short peptides (<7AA) excluded
NIST_mouse_IT_2009-12-10.msp.zip 267M 2010-06-30 
3d02b5b06d9c2a9e51e9049a306cc46c
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Mus musculus)
NIST_mouse_IT_2009-12-10.nist.zip 512M 2010-06-30 
9d7cc9e461280511ad83b8da2abbaf47
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Mus musculus) -- NIST format
NIST_mouse_IT_2009-12-10_7AA.splib.zip 628M 2010-06-30 
5a2a4afda9b585f142be44074758ba08
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Mus musculus) -- SpectraST format with short peptides (<7AA) excluded
NIST_msmegmatis_IT_2010-03-31.msp.zip 7.7M 2010-06-30 
aad3ab5a7e048427bcb180c40eff6953
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: M. smegmatis)
NIST_msmegmatis_IT_2010-03-31.nist.zip 17M 2010-06-30 
36b4cf2330ffee780498451eab3fe7ba
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: M. smegmatis) -- NIST format
NIST_msmegmatis_IT_2010-03-31_7AA.splib.zip 19M 2010-06-30 
679332107b1747eb2af44720f818724c
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: M. smegmatis) -- SpectraST format with short peptides (<7AA) excluded
NIST_nci_stdmix_IT_2009-11-24.msp.zip 6.8M 2010-06-30 
7f0d201c59ab76d61697394084afc85c
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: NCI standard mix)
NIST_nci_stdmix_IT_2009-11-24.nist.zip 14M 2010-06-30 
098a563d3cf22b6de025cb1c57b88023
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: NCI standard mix) -- NIST format
NIST_nci_stdmix_IT_2009-11-24_7AA.splib.zip 16M 2010-06-30 
cb896a7c7a4ffea0c9f5c72e1f8761d4
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: NCI standard mix) -- SpectraST format with short peptides (<7AA) excluded
NIST_rat_IT_v3.0_2009-05-21.msp.zip 35M 2009-05-29 
dbe031cbfc32953517097ef4863103de
NIST Library of Peptide Ion Fragmentation Spectra (Rattus norvegicus)
NIST_rat_IT_v3.0_2009-05-21.nist.zip 64M 2009-05-29 
f2fa6b41ee3e8e75e36faa18d77a11a2
NIST Library of Peptide Ion Fragmentation Spectra (Rattus norvegicus) -- NIST format
NIST_rat_IT_v3.0_2009-05-21_7AA.splib.zip 82M 2009-05-29 
a2fbf212a27af4ff82b561d5a1124c74
NIST Library of Peptide Ion Fragmentation Spectra (Rattus norvegicus) -- SpectraST format with short peptides (<7AA) excluded
NIST_sigmaups1_IT_v3.0_2008-07-09.msp.zip 5.6M 2009-05-29 
2726c54c75d9538df63ff3eebcab30b7
NIST library for the Sigma UPS1 proteomics standard [link]
NIST_sigmaups1_IT_v3.0_2008-07-09.nist.zip 11M 2009-05-29 
6e0d89bddbf3c51e89e20c68f048fe87
NIST library for the Sigma UPS1 proteomics standard [link] -- NIST format
NIST_sigmaups1_IT_v3.0_2008-07-09_7AA.splib.zip 14M 2009-05-29 
77b4a4d0dde8462283e6757ac41fffc1
NIST library for the Sigma UPS1 proteomics standard [link] -- SpectraST format with short peptides (<7AA) excluded
NIST_yeast_IT_2009-10-19.msp.zip 146M 2010-06-30 
6b4d6fd68afe05ad9f0d388fa30241f0
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Saccharomyces cerevisiae)
NIST_yeast_IT_2009-10-19.nist.zip 286M 2010-06-30 
3941738509fcfedbdd9bfb71582f3ef4
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Saccharomyces cerevisiae) -- NIST format
NIST_yeast_IT_2009-10-19_7AA.splib.zip 346M 2010-06-30 
041c0840e9d44b353824abc1caf1012e
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Saccharomyces cerevisiae) -- SpectraST format with short peptides (<7AA) excluded
NIST_yeast_QTOF_2009-11-25.msp.zip 2.9M 2010-06-30 
c0aa5a208adb1d3c7b0f4ff6cf1b9da4
NIST Library of Peptide Ion Fragmentation Spectra (QTOF: Saccharomyces cerevisiae)
NIST_yeast_QTOF_2009-11-25.nist.zip 6.4M 2010-06-30 
4d6dc7693ba9059b1b4c8d78ead85b4e
NIST Library of Peptide Ion Fragmentation Spectra (QTOF: Saccharomyces cerevisiae) -- NIST format
NIST_yeast_QTOF_2009-11-25_7AA.splib.zip 7.0M 2010-06-30 
c51a1a0a1ba0c6e556b6f83d5cb3205c
NIST Library of Peptide Ion Fragmentation Spectra (QTOF: Saccharomyces cerevisiae) -- SpectraST format with short peptides (<7AA) excluded
[ + ] Show old/archived libraries
NIST_drosophila_IT_v3.0_2008-07-14.msp.zip 167M 2009-05-29 
fd27463057c6e92bcf264202155d3178
NIST Library of Peptide Ion Fragmentation Spectra (Drosophila) -- NIST format
NIST_drosophila_IT_v3.0_2008-07-14.nist.zip 306M 2009-05-29 
efdc2ad837e1e6fdd0d2e5da7381c913
NIST Library of Peptide Ion Fragmentation Spectra (Drosophila) -- NIST format
NIST_drosophila_IT_v3.0_2008-07-14_7AA.splib.zip 389M 2009-05-29 
79f1e1f6c736d7126d00ab74c91d638c
NIST Library of Peptide Ion Fragmentation Spectra (Drosophila) -- SpectraST format with short peptides (<7AA) excluded
NIST_ecoli_IT_v3.0_2009-05-21.msp.zip 82M 2009-05-29 
b30d3a5a20b6c3908fc7d86ce0ce3bc2
NIST Library of Peptide Ion Fragmentation Spectra (Escherichia coli)
NIST_ecoli_IT_v3.0_2009-05-21.nist.zip 151M 2009-05-29 
638da0d26c13af9b23271ded24f229e9
NIST Library of Peptide Ion Fragmentation Spectra (Escherichia coli) -- NIST format
NIST_ecoli_IT_v3.0_2009-05-21.splib.zip 197M 2009-05-29 
beebe16cb514caccfcfd472f4d4fca35
NIST Library of Peptide Ion Fragmentation Spectra (Escherichia coli) -- SpectraST format
NIST_hsa_IT_v3.0_2009-05-06.msp.zip 4.4M 2009-05-29 
bf91cbb5a0b89fbd1ba71e5a1d8273fa
NIST Library of Peptide Ion Fragmentation Spectra (Human Serum Albumin)
NIST_hsa_IT_v3.0_2009-05-06.nist.zip 8.3M 2009-05-29 
8f3afb635fb8283291b463576c0be08a
NIST Library of Peptide Ion Fragmentation Spectra (Human Serum Albumin) -- NIST format
NIST_hsa_IT_v3.0_2009-05-06.splib.zip 12M 2009-05-29 
425ea76d849a6b8d34bafc2e020f9641
NIST Library of Peptide Ion Fragmentation Spectra (Human Serum Albumin) -- SpectraST format
NIST_human_IT_v3.0_2009-02-04.msp.zip 461M 2009-05-29 
d48df03a8fb5382cc632b98951700156
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Homo sapiens)
NIST_human_IT_v3.0_2009-02-04.nist.zip 803M 2009-05-29 
e8ad2559454401fa39be44ae0506b4f5
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Homo sapiens) -- NIST format
NIST_human_IT_v3.0_2009_02_04_7AA.splib.zip 1.1G 2009-05-29 
feef5a7e19d0b7934b3fcdfca8dbd8c2
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Homo sapiens) -- SpectraST format with short peptides (<7AA) excluded
NIST_human_QTOF_v3.0_2008-07-22.msp.zip 16M 2009-05-29 
481623b4de7a411fc7f183f23548844c
NIST Library of Peptide Ion Fragmentation Spectra (QTOF: Homo sapiens)
NIST_human_QTOF_v3.0_2008-07-22.nist.zip 31M 2009-05-29 
71fffdbf420114770385a51435753aa5
NIST Library of Peptide Ion Fragmentation Spectra (QTOF: Homo sapiens) -- NIST format
NIST_human_QTOF_v3.0_2008-07-22_7AA.splib.zip 39M 2009-05-29 
9cffc0d0fb59df58e6e54f6d60a69708
NIST Library of Peptide Ion Fragmentation Spectra (QTOF: Homo sapiens) -- SpectraST format with short peptides (<7AA) excluded
NIST_mouse_IT_v3.0_2009-04-29.msp.zip 228M 2009-05-29 
bf52536ee1ab689623de10c87b61b511
NIST Library of Peptide Ion Fragmentation Spectra (Mus musculus)
NIST_mouse_IT_v3.0_2009-04-29.nist.zip 408M 2009-05-29 
9f6db19bbb33a0a273f61e88c4eb252e
NIST Library of Peptide Ion Fragmentation Spectra (Mus musculus) -- NIST format
NIST_mouse_IT_v3.0_2009-04-29_7AA.splib.zip 530M 2009-05-29 
851f6df604fda79d8fad4858e798947f
NIST Library of Peptide Ion Fragmentation Spectra (Mus musculus) -- SpectraST format with short peptides (<7AA) excluded
NIST_nci_stdmix_IT_v3.0_2008-09-26.msp.zip 5.8M 2009-05-29 
1cd8683680913ab2a84c770e0d97e013
NIST Library of Peptide Ion Fragmentation Spectra (NCI standard mix)
NIST_nci_stdmix_IT_v3.0_2008-09-26.nist.zip 13M 2009-05-29 
fad235e638b3681df552c5aa4f7b8e3c
NIST Library of Peptide Ion Fragmentation Spectra (NCI standard mix) -- NIST format
NIST_nci_stdmix_IT_v3.0_2008-09-26.splib.zip 16M 2009-05-29 
f7611ac6de5835c3adc062a624af1bed
NIST Library of Peptide Ion Fragmentation Spectra (NCI standard mix) -- SpectraST format
NIST_yeast_IT_v3.0_2009-05-04.msp.zip 149M 2009-05-29 
970bc837b99be88c36d28827b9e1841c
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Saccharomyces cerevisiae)
NIST_yeast_IT_v3.0_2009-05-04.nist.zip 263M 2009-05-29 
3e05554126a149bf2c6bf3679ac4f640
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Saccharomyces cerevisiae) -- SpectraST format
NIST_yeast_IT_v3.0_2009-05-04_7AA.splib.zip 346M 2009-05-29 
d7e1e39cec99c8170986f0e90e8d4036
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Saccharomyces cerevisiae) -- SpectraST format with short peptides (<7AA) excluded
NIST_yeast_QTOF_v3.0_2009-05-06.msp.zip 2.2M 2009-05-29 
20f292d1fa76a075c78b0bdd3629a464
NIST Library of Peptide Ion Fragmentation Spectra (QTOF: Saccharomyces cerevisiae)
NIST_yeast_QTOF_v3.0_2009-05-06.nist.zip 4.4M 2009-05-29 
9462ae9081d6ad6adca9cc8370e008cf
NIST Library of Peptide Ion Fragmentation Spectra (QTOF: Saccharomyces cerevisiae) -- NIST format
NIST_yeast_QTOF_v3.0_2009-05-06_7AA.splib.zip 5.0M 2009-05-29 
75fc33277ca980610be8c98e2219cbe3
NIST Library of Peptide Ion Fragmentation Spectra (QTOF: Saccharomyces cerevisiae) -- SpectraST format with short peptides (<7AA) excluded
NIST_aurum_TOFTOF_v2.0_2008-07-15.msp.zip 488K 2008-08-13 
9d978899664a58a8a9dca3e9291efa3e
NIST Library of Peptide Ion Fragmentation Spectra (Aurum Dataset of purchased proteins)
NIST_aurum_TOFTOF_v2.0_2008-07-15.splib.zip 536K 2008-08-13 
c88e5583565d31e7e5bfd240e44a5598
NIST Library of Peptide Ion Fragmentation Spectra (Aurum Dataset) -- SpectraST format
NIST_ecoli_IT_v2.0_2008-07-11.msp.zip 70M 2008-07-15 
702d2afab4e4a50dcf98feff3175ea3d
NIST Library of Peptide Ion Fragmentation Spectra (Escherichia coli)
NIST_ecoli_IT_v2.0_2008-07-11.splib.zip 77M 2008-07-15 
6950a48bb5bfa9dc34ce4bd9eb38eefe
NIST Library of Peptide Ion Fragmentation Spectra (Escherichia coli) -- SpectraST format
NIST_human_IT_v2.0_2008-07-11.msp.zip 389M 2008-07-15 
5157623e6440e43dedf0d80f0a3cef5f
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Homo sapiens)
NIST_human_IT_v2.0_2008-07-11.splib.zip 421M 2008-07-15 
02adcb16a42a71a53f41bc9eb3eaf2f8
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Homo sapiens) -- SpectraST format
NIST_human_QTOF_v2.0_2008-07-22.msp.zip 16M 2008-08-13 
916fc8e21fe901d8b44ca2f75bce69d5
NIST Library of Peptide Ion Fragmentation Spectra (QTOF: Homo sapiens)
NIST_human_QTOF_v2.0_2008-07-22.splib.zip 17M 2008-08-13 
2998abc7b93cc56f5c9718d2cbfaf456
NIST Library of Peptide Ion Fragmentation Spectra (QTOF: Homo sapiens) -- SpectraST format
NIST_mouse_IT_v2.0_2008-06-30.msp.zip 67M 2008-07-15 
eb12f2a46a4f964684d4641e3119f8e0
NIST Library of Peptide Ion Fragmentation Spectra (Mus musculus)
NIST_mouse_IT_v2.0_2008-06-30.splib.zip 73M 2008-07-15 
b27e30912fecfa62c95827e04be4ff95
NIST Library of Peptide Ion Fragmentation Spectra (Mus musculus) -- SpectraST format
NIST_rat_IT_v2.0_2008-05-29.msp.zip 20M 2008-07-15 
1cd2152cddf1d7591365b5865fb6a406
NIST Library of Peptide Ion Fragmentation Spectra (Rattus norvegicus)
NIST_rat_IT_v2.0_2008-05-29.splib.zip 22M 2008-07-15 
2d7a53e70bb858136b4c4fc30da9bfaa
NIST Library of Peptide Ion Fragmentation Spectra (Rattus norvegicus) -- SpectraST format
NIST_sigmaups1_IT_v2.0_2008-07-09.msp.zip 5.5M 2008-07-15 
3b0ec5ca778e6f436c0e1b9ded6e8b72
NIST library for the Sigma UPS1 proteomics standard [link]
NIST_sigmaups1_IT_v2.0_2008-07-09.splib.zip 6.0M 2008-07-15 
da4dcb4526d485452693b65a5e44ed46
NIST library for the Sigma UPS1 proteomics standard [link] -- SpectraST format
NIST_yeast_IT_v2.0_2008-07-11.msp.zip 135M 2008-07-15 
234d8c2cd4a71e055216e72ab66b6217
NIST Library of Peptide Ion Fragmentation Spectra (Saccharomyces cerevisiae)
NIST_yeast_IT_v2.0_2008-07-11.splib.zip 142M 2008-07-15 
4af25221a524b4cdb6abae792d48054e
NIST Library of Peptide Ion Fragmentation Spectra (Saccharomyces cerevisiae) -- SpectraST format
NIST_yeast_QTOF_v2.0_2008-04-30.msp.zip 2.1M 2008-08-13 
8fe1dcc654456eec24a87c411ff7fdcb
NIST Library of Peptide Ion Fragmentation Spectra (QTOF: Saccharomyces cerevisiae)
NIST_yeast_QTOF_v2.0_2008-04-30.splib.zip 2.1M 2008-08-13 
c606a540d6ff9c51683f2c3785d04684
NIST Library of Peptide Ion Fragmentation Spectra (QTOF: Saccharomyces cerevisiae) -- SpectraST format
NIST_msmegmatis_IT_v1.0_2006-06-06.msp.zip 11M 2008-07-17 
61efedf084e3c9242cab91f9f33ed041
NIST Library of Peptide Ion Fragmentation Spectra (Mycobacterium smegmatis)
NIST_msmegmatis_IT_v1.0_2006-06-06.splib.zip 13M 2008-07-17 
4bbe439cfd853dbc8a6cb8e25e0b2b0a
NIST Library of Peptide Ion Fragmentation Spectra (Mycobacterium smegmatis) -- SpectraST format
NIST_dradiodurans_IT_v1.0_2006-06-06.msp.zip 23M 2008-07-17 
ffde4a862a23fa89956ffeb9d46b4c99
NIST Library of Peptide Ion Fragmentation Spectra (Deinococcus radiodurans)
NIST_dradiodurans_IT_v1.0_2006-06-06.splib.zip 26M 2008-07-17 
e3d61bd772c224ce92c0e4fa16938575
NIST Library of Peptide Ion Fragmentation Spectra (Deinococcus radiodurans) -- SpectraST format
yeast.msp.zip 110MB 2006-12-13 
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NIST Library of Peptide Ion Fragmentation Spectra (Saccharomyces Cerevisae)
yeast_consensus.splib 152MB 2006-12-13 
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NIST Library of Peptide Ion Fragmentation Spectra (Saccharomyces Cerevisae) -- SpectraST format
human.msp.zip 137MB 2006-12-13 
d7c7baba10ef6ab2592665689e3dc4ef
NIST Library of Peptide Ion Fragmentation Spectra (Human)
human_consensus.splib 193MB 2006-12-13 
a2b8dc52513348ed3850d9d8a20d2b25
NIST Library of Peptide Ion Fragmentation Spectra (Human) -- SpectraST format
NIST_PepLib_6_06.doc 240kB 2006-12-13 
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Documentation for NIST Libraries (doc)
NIST_PepLib_6_06.pdf 114kB 2006-12-13 
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Documentation for NIST Libraries (pdf)
Read_Me_First.txt 4kB 2006-12-13 
0020d9ae92dbdc0d19cc13a529548e0a
Read this file first! For more info on the files that follow.
NIST_Pep_Setup.zip 467MB 2006-12-13 
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NIST MS Search and complete NIST Library of Peptide Ion Fragmentation Spectra setup
NIST_Pep_Demo_Setup.zip 22MB 2006-12-13 
75ecd2186b8d050445be8652bc8147aa
Demo (abbreviated) version of the above
NIST_PEPLIB_TXT.ZIP 306MB 2006-12-13 
9efff01550fbb9dc32a1efa80326cb85
Text version of the NIST Library of Peptide Ion Fragmentation Spectra with protein sequences
NIST_PEP_SRCH_ENGINE.ZIP 3MB 2006-12-13 
8ee0c07e118d134ec38f062f0ee971dc
NIST MS/MS Peptide Libraries DLL version 2.1.1. Sample code and documentation for using the DLL


   GPM

The Global Proteome Database builds consensus Annotated Spectral Libraries (ASL) for several organisms (human, yeast, mouse, dog, cow, etc), for use with the X!Hunter application, and are available for download via ftp. Please note that these libraries are not suitable to be used with SpectraST.



   BiblioSpec

Libraries can be built using the BlibBuild program that is part of the software distribution. Pre-built reference libraries should be available for download from this link.

* To make sure that you have a complete and uncorrupted download, please verify that the md5 checksum of each downloaded file matches the posted value on this website. (On most Unix-type systems, you can use the md5sum utility: md5sum filename).
© 2009, Institute for Systems Biology, All Rights Reserved