Welcome to the Spectrum Library Central, hosted at PeptideAtlas.
We provide a list and links to Spectrum Libraries built specifically for
spectrum library searching of tandem mass-spec data.
Spectral Library Search Projects
Spectral library searching, unlike sequence database searches,
involve finding the best match of an acquired MS/MS spectrum to
a library of pre-searched spectra for which the sequences have been
determined. This approach can be hundreds of times faster
than traditional searching, with comparable or better accuracy.
The National Institute of Standards and Technology (NIST)
distributes an updated version of their
NIST MS Search Program for Windows,
which includes the ability to perform Peptide searches via spectrum matching.
The NIST Peptide Library Search Engine is also available from the
NIST download section below.
Quality-filtered consensus spectral libraries of Caenorhabditis elegans peptides enriched for phosphorylation. (Bodenmiller et al. (2008) Nature Biotechnology 26, 1339-1340 )
ISB_Hs-phospho_20080428.zip
21MB
2008-04-28
036e4907522c47775c55c204f3c47c56
Quality-filtered consensus spectral libraries of Homo sapiens peptides enriched for phosphorylation. (Bodenmiller et al. (2008) Nature Biotechnology 26, 1339-1340) I recognize that this data is the property of Cell Signaling Technology and agree not to use it for any commercial purpose whatsoever without their express written consent.
ISB_Sc-phospho_20080313.zip
94MB
2008-03-13
d00aa125f77f95f9911c1c365f9ddd0f
Quality-filtered consensus spectral libraries of Saccharomyces cerevisiae peptides enriched for phosphorylation. (Bodenmiller et al. (2008) Nature Biotechnology 26, 1339-1340 )
SpectraST_2008_07_01.zip
27MB
2008-07-01
545600349c50613c03c3c5a65974ffec
Stand-alone Windows native version of SpectraST (also available as part of the TPP distribution)
These libraries are built by S. Stein at NIST; more information and related software can be found at peptide.nist.gov. The Institute for Systems Biology asks that anyone wishing to download any of the NIST Libraries of Peptide Fragmentation Spectra read the following paragraph and acknowledge before downloading:
"I recognize that this data collection, the NIST Library of Peptide Fragmentation Spectra, is copyrighted by the Department of Commerce, U.S. Government and shall not be redistributed in any manner without a valid Distribution Agreement. To receive such an agreement, contact the Standard Reference Data Program, National Institute of Standards and Technology, at data*at*nist.gov or 301-975-2008."
File
Size
Date
md5 checksum*
Download
NIST_aurum_TOFTOF_v3.0_2008-07-15.msp.zip
508K
2009-05-29
ab03a7c84eb19615e75d2346061bfe49
NIST Library of Peptide Ion Fragmentation Spectra (Aurum Dataset of purchased proteins)
NIST_aurum_TOFTOF_v3.0_2008-07-15.nist.zip
1.2M
2009-05-29
10a97de56f17431c7525c2e2689b428b
NIST Library of Peptide Ion Fragmentation Spectra (Aurum Dataset) -- NIST format
NIST_aurum_TOFTOF_v3.0_2008-07-15_7AA.splib.zip
1.1M
2009-05-29
4e724b743219531036d4878e08591c10
NIST Library of Peptide Ion Fragmentation Spectra (Aurum Dataset) -- SpectraST format with short peptides (<7AA) excluded
NIST_bsa_IT_v3.0_2009-05-06.msp.zip
1.6M
2009-05-29
dc92b5aa378c7eee2d57e93a22702e97
NIST Library of Peptide Ion Fragmentation Spectra (bsa)
NIST_bsa_IT_v3.0_2009-05-06.nist.zip
3.2M
2009-05-29
86115b19767f2b1cc9be368d9f43d4a6
NIST Library of Peptide Ion Fragmentation Spectra (bsa) -- NIST format
NIST_bsa_IT_v3.0_2009-05-06.splib.zip
3.8M
2009-05-29
16fc779ca6179048ef67b39ca1ec29b0
NIST Library of Peptide Ion Fragmentation Spectra (bsa) -- SpectraST format
NIST_chicken_IT_v3.0_2009-04-15.msp.zip
7.9M
2009-05-29
f44fb2da409780190e26c0827b1e1c9a
NIST Library of Peptide Ion Fragmentation Spectra (Chicken)
NIST_chicken_IT_v3.0_2009-04-15.nist.zip
15M
2009-05-29
d58f2b09e8180ab0c56f4010edbc3667
NIST Library of Peptide Ion Fragmentation Spectra (Chicken) -- NIST format
NIST_chicken_IT_v3.0_2009-04-15_7AA.splib.zip
19M
2009-05-29
5c1656e7d10f74638c26748b7a595c33
NIST Library of Peptide Ion Fragmentation Spectra (Chicken) -- SpectraST format with short peptides (<7AA) excluded
NIST_drosophila_IT_v3.0_2008-07-14.msp.zip
167M
2009-05-29
fd27463057c6e92bcf264202155d3178
NIST Library of Peptide Ion Fragmentation Spectra (Drosophila) -- NIST format
NIST_drosophila_IT_v3.0_2008-07-14.nist.zip
306M
2009-05-29
efdc2ad837e1e6fdd0d2e5da7381c913
NIST Library of Peptide Ion Fragmentation Spectra (Drosophila) -- NIST format
NIST_drosophila_IT_v3.0_2008-07-14_7AA.splib.zip
389M
2009-05-29
79f1e1f6c736d7126d00ab74c91d638c
NIST Library of Peptide Ion Fragmentation Spectra (Drosophila) -- SpectraST format with short peptides (<7AA) excluded
NIST_ecoli_IT_v3.0_2009-05-21.msp.zip
82M
2009-05-29
b30d3a5a20b6c3908fc7d86ce0ce3bc2
NIST Library of Peptide Ion Fragmentation Spectra (Escherichia coli)
NIST_ecoli_IT_v3.0_2009-05-21.nist.zip
151M
2009-05-29
638da0d26c13af9b23271ded24f229e9
NIST Library of Peptide Ion Fragmentation Spectra (Escherichia coli) -- NIST format
NIST_ecoli_IT_v3.0_2009-05-21.splib.zip
197M
2009-05-29
beebe16cb514caccfcfd472f4d4fca35
NIST Library of Peptide Ion Fragmentation Spectra (Escherichia coli) -- SpectraST format
NIST_hsa_IT_v3.0_2009-05-06.msp.zip
4.4M
2009-05-29
bf91cbb5a0b89fbd1ba71e5a1d8273fa
NIST Library of Peptide Ion Fragmentation Spectra (Human Serum Albumin)
NIST_hsa_IT_v3.0_2009-05-06.nist.zip
8.3M
2009-05-29
8f3afb635fb8283291b463576c0be08a
NIST Library of Peptide Ion Fragmentation Spectra (Human Serum Albumin) -- NIST format
NIST_hsa_IT_v3.0_2009-05-06.splib.zip
12M
2009-05-29
425ea76d849a6b8d34bafc2e020f9641
NIST Library of Peptide Ion Fragmentation Spectra (Human Serum Albumin) -- SpectraST format
NIST_human_b2mg_IT_v3.0_2009-05-06.msp.zip
260K
2009-05-29
b23ddec5023df08c9b074359497c4694
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Homo sapiens / Beta-2-microglobulin)
NIST_human_b2mg_IT_v3.0_2009-05-06.nist.zip
560K
2009-05-29
aafe89afaced595a9701abd9b8cace34
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Homo sapiens / Beta-2-microglobulin) -- NIST format
NIST_human_b2mg_IT_v3.0_2009-05-06_7AA.splib.zip
624K
2009-05-29
ba7e4f59afc5eec2a6b9ddd42bf8e4a2
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Homo sapiens / Beta-2-microglobulin) -- SpectraST format with short peptides (<7AA) excluded
NIST_human_crp_IT_v3.0_2009-05-06.msp.zip
108K
2009-05-29
8e0c0ebdafa49f0637e23a670cb62dac
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Homo sapiens / C-reactive protein)
NIST_human_crp_IT_v3.0_2009-05-06.nist.zip
244K
2009-05-29
9116eb32102f28138a83772a339200bc
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Homo sapiens / C-reactive protein) -- NIST format
NIST_human_crp_IT_v3.0_2009-05-06_7AA.splib.zip
232K
2009-05-29
dfbda3bd315baef7aa8f60a1312bbe50
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Homo sapiens / C-reactive protein) -- SpectraST format with short peptides (<7AA) excluded
NIST_human_IT_v3.0_2009-02-04.msp.zip
461M
2009-05-29
d48df03a8fb5382cc632b98951700156
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Homo sapiens)
NIST_human_IT_v3.0_2009-02-04.nist.zip
803M
2009-05-29
e8ad2559454401fa39be44ae0506b4f5
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Homo sapiens) -- NIST format
NIST_human_IT_v3.0_2009_02_04_7AA.splib.zip
1.1G
2009-05-29
feef5a7e19d0b7934b3fcdfca8dbd8c2
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Homo sapiens) -- SpectraST format with short peptides (<7AA) excluded
NIST_human_QTOF_v3.0_2008-07-22.msp.zip
16M
2009-05-29
481623b4de7a411fc7f183f23548844c
NIST Library of Peptide Ion Fragmentation Spectra (QTOF: Homo sapiens)
NIST_human_QTOF_v3.0_2008-07-22.nist.zip
31M
2009-05-29
71fffdbf420114770385a51435753aa5
NIST Library of Peptide Ion Fragmentation Spectra (QTOF: Homo sapiens) -- NIST format
NIST_human_QTOF_v3.0_2008-07-22_7AA.splib.zip
39M
2009-05-29
9cffc0d0fb59df58e6e54f6d60a69708
NIST Library of Peptide Ion Fragmentation Spectra (QTOF: Homo sapiens) -- SpectraST format with short peptides (<7AA) excluded
NIST_mouse_IT_v3.0_2009-04-29.msp.zip
228M
2009-05-29
bf52536ee1ab689623de10c87b61b511
NIST Library of Peptide Ion Fragmentation Spectra (Mus musculus)
NIST_mouse_IT_v3.0_2009-04-29.nist.zip
408M
2009-05-29
9f6db19bbb33a0a273f61e88c4eb252e
NIST Library of Peptide Ion Fragmentation Spectra (Mus musculus) -- NIST format
NIST_mouse_IT_v3.0_2009-04-29_7AA.splib.zip
530M
2009-05-29
851f6df604fda79d8fad4858e798947f
NIST Library of Peptide Ion Fragmentation Spectra (Mus musculus) -- SpectraST format with short peptides (<7AA) excluded
NIST_nci_stdmix_IT_v3.0_2008-09-26.msp.zip
5.8M
2009-05-29
1cd8683680913ab2a84c770e0d97e013
NIST Library of Peptide Ion Fragmentation Spectra (NCI standard mix)
NIST_nci_stdmix_IT_v3.0_2008-09-26.nist.zip
13M
2009-05-29
fad235e638b3681df552c5aa4f7b8e3c
NIST Library of Peptide Ion Fragmentation Spectra (NCI standard mix) -- NIST format
NIST_nci_stdmix_IT_v3.0_2008-09-26.splib.zip
16M
2009-05-29
f7611ac6de5835c3adc062a624af1bed
NIST Library of Peptide Ion Fragmentation Spectra (NCI standard mix) -- SpectraST format
NIST_rat_IT_v3.0_2009-05-21.msp.zip
35M
2009-05-29
dbe031cbfc32953517097ef4863103de
NIST Library of Peptide Ion Fragmentation Spectra (Rattus norvegicus)
NIST_rat_IT_v3.0_2009-05-21.nist.zip
64M
2009-05-29
f2fa6b41ee3e8e75e36faa18d77a11a2
NIST Library of Peptide Ion Fragmentation Spectra (Rattus norvegicus) -- NIST format
NIST_rat_IT_v3.0_2009-05-21_7AA.splib.zip
82M
2009-05-29
a2fbf212a27af4ff82b561d5a1124c74
NIST Library of Peptide Ion Fragmentation Spectra (Rattus norvegicus) -- SpectraST format with short peptides (<7AA) excluded
NIST_sigmaups1_IT_v3.0_2008-07-09.msp.zip
5.6M
2009-05-29
2726c54c75d9538df63ff3eebcab30b7
NIST library for the Sigma UPS1 proteomics standard [link]
NIST_sigmaups1_IT_v3.0_2008-07-09.nist.zip
11M
2009-05-29
6e0d89bddbf3c51e89e20c68f048fe87
NIST library for the Sigma UPS1 proteomics standard [link] -- NIST format
NIST_sigmaups1_IT_v3.0_2008-07-09_7AA.splib.zip
14M
2009-05-29
77b4a4d0dde8462283e6757ac41fffc1
NIST library for the Sigma UPS1 proteomics standard [link] -- SpectraST format with short peptides (<7AA) excluded
NIST_yeast_IT_v3.0_2009-05-04.msp.zip
149M
2009-05-29
970bc837b99be88c36d28827b9e1841c
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Saccharomyces cerevisiae)
NIST_yeast_IT_v3.0_2009-05-04.nist.zip
263M
2009-05-29
3e05554126a149bf2c6bf3679ac4f640
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Saccharomyces cerevisiae) -- SpectraST format
NIST_yeast_IT_v3.0_2009-05-04_7AA.splib.zip
346M
2009-05-29
d7e1e39cec99c8170986f0e90e8d4036
NIST Library of Peptide Ion Fragmentation Spectra (Ion Trap: Saccharomyces cerevisiae) -- SpectraST format with short peptides (<7AA) excluded
NIST_yeast_QTOF_v3.0_2009-05-06.msp.zip
2.2M
2009-05-29
20f292d1fa76a075c78b0bdd3629a464
NIST Library of Peptide Ion Fragmentation Spectra (QTOF: Saccharomyces cerevisiae)
NIST_yeast_QTOF_v3.0_2009-05-06.nist.zip
4.4M
2009-05-29
9462ae9081d6ad6adca9cc8370e008cf
NIST Library of Peptide Ion Fragmentation Spectra (QTOF: Saccharomyces cerevisiae) -- NIST format
NIST_yeast_QTOF_v3.0_2009-05-06_7AA.splib.zip
5.0M
2009-05-29
75fc33277ca980610be8c98e2219cbe3
NIST Library of Peptide Ion Fragmentation Spectra (QTOF: Saccharomyces cerevisiae) -- SpectraST format with short peptides (<7AA) excluded
The Global Proteome Database builds consensus Annotated Spectral Libraries (ASL) for several organisms (human, yeast, mouse, dog, cow, etc), for use with the X!Hunter application, and are available for download via ftp. Please note that these libraries are not suitable to be used with SpectraST.
Libraries can be built using the BlibBuild program that is part of the software distribution. Pre-built reference libraries should be available for download from this link.
* To make sure that you have a complete and uncorrupted download, please verify that the
md5 checksum of each downloaded file matches the posted value on this website. (On most Unix-type
systems, you can use the md5sum utility: md5sum filename).