Spectrum Library Central at PeptideAtlas

Welcome to the Spectrum Library Central, hosted at PeptideAtlas. We provide a list and links to Spectrum Libraries built specifically for spectrum library searching of tandem mass-spec data.

Spectral Library Search Projects

Spectral library searching, unlike sequence database searches, involve finding the best match of an acquired MS/MS spectrum to a library of pre-searched spectra for which the sequences have been determined. This approach can be hundreds of times faster than traditional searching, with comparable or better accuracy.

SpectraST	Developed at ISB by H.Lam. Included as part of the Trans-Proteomic Pipeline (TPP) distribution. An online query form is also available. For more information, please consult the SpectraST information page
NIST MS Search	The National Institute of Standards and Technology (NIST) distributes an updated version of their NIST MS Search Program for Windows, which includes the ability to perform Peptide searches via spectrum matching.
X! Hunter	Developed by the The Global Proteome Machine Organization. Online query forms for submitting files or spectra are no longer available.
BlibSearch	Part of the BiblioSpec suite, developed at the MacCoss Lab at the University of Washington.
Pepitome	A spectral library search engine which employs statistical systems in its scoring algorithm, developed at the Tabb Lab at Vanderbilt University.
Bonanza	A spectral comparison algorithm developed by the Andrews Lab at the University of Michigan. (No website or data yet).
Tremolo	Spectral library search tool that leverages the Spectral Library Generating Function (SLGF) concept to identify spectrum-spectrum matches. The SLGF models the variability of replicate spectra as compared to reference library spectra. Developed at the Center for Computational Mass Spectrometry at UCSD.

Download Spectrum Libraries

Please visit ProteomeCentral to explore and download spectrum libraries from various sources.