| Metadata |
| datasetIdentifier | PASS00007 |
| datasetType | SRM |
| submitter | Ruth Hüttenhain <huettenhain@imsb.biol.ethz.ch> |
| submitter_organization | IMSB |
| lab_head_full_name | Ruedi Aebersold |
| lab_head_email | aebersold@imsb.biol.ethz.ch |
| lab_head_organization | IMSB |
| lab_head_country | Switzerland |
| datasetTag | CAP_urine |
| datasetTitle | Cancer associated proteins urine |
| publicReleaseDate | 2012-05-10 00:00:00 |
| finalizedDate | 2011-09-24 09:25:25 |
| summary | A list of cancer-associated proteins was targeted in human urine by label-free SRM. The data was analyzed using mProphet. |
| contributors | Ruth Huettenhain |
| publication | unpublished |
| growth | |
| treatment | |
| extraction | Protein precipitation was achieved by adding TCA to 10 mL of urine to a final concentration of 6%. The sample was mixed and incubated at 4C for 2 h followed by centrifugation at 14000xg for 15 min. The supernatant was removed and the pellet was washed twice with 100% ice-cold acetone to remove all interfering compounds. |
| separation | Peptides were separated in 45 minutes by a linear gradient of acetonitrile (5 - 35%) and water, containing 0.1% formic acid at a flow rate of 300 nL min-1. |
| digestion | Trypsin digestion |
| acquisition | For target detection in urine, peptide preparations were analyzed on a TSQ Vantage triple-quadrupole mass spectrometer (Thermo Fisher) in scheduled SRM acquisition mode. SRM acquisition was performed with Q1 and Q3 operated at resolution of 0.7 m/z half maximum peak width, with a retention time window of 240 s and a cycle time of 2.0 s. |
| informatics | mProphet analysis |
| instruments | Thermo TSQ Vantage |
| species | Human |
| massModifications | static: C+57.021464 |
