| Metadata |
| datasetIdentifier | PASS00036 |
| datasetType | SRM |
| submitter | Christina Ludwig <ludwig@imsb.biol.ethz.ch> |
| submitter_organization | IMSB |
| lab_head_full_name | Ruedi Aebersold |
| lab_head_email | aebersold@imsb.biol.ethz.ch |
| lab_head_organization | IMSB |
| lab_head_country | Switzerland |
| datasetTag | phospho_yeast_metabo |
| datasetTitle | Regulation of yeast central metabolism by enzyme phosphorylation - quantification of phosphorylation site changes in Pda1, Pfk2 and Gpd1 |
| publicReleaseDate | 2012-06-01 00:00:00 |
| finalizedDate | |
| summary | As a frequent post-translational modification, reversible protein phosphorylation regulates many cellular processes. Although several hundred phosphorylation sites have been mapped to metabolic enzymes in Saccharomyces cerevisiae, functionality was demonstrated for few of them. Here we identify enzyme phosphorylation events that are important in regulating the functioning of the network of 204 enzymes in yeast central carbon and amino acid metabolism. Specifically, we identified 35 differently phosphorylated enzymes during growth under five conditions, and combined these data with previously determined protein abundances and flux estimates. Correlation of intracellular fluxes with total or phosphoprotein abundances allowed us to assign function to seven previously unknown phosphorylation events, doubling the number of described functional phosphoenzymes in central carbon and amino acid metabolism. Finally, we validated the functional impact of selected enzyme phosphosites (Pda1, Pfk2, Gpd1) on the functioning of the entire metabolic network through genetic removal of phosphosites and by specific phosphosite quantification using targeted mass spectrometry. |
| contributors | Christina Ludwig
Ana Paula Oliveira
Paola Picotti
Ruedi Aebersold
Uwe Sauer |
| publication | this work is not published yet |
| growth | |
| treatment | |
| extraction | |
| separation | |
| digestion | |
| acquisition | |
| informatics | |
| instruments | TSQ Vantage |
| species | Saccharomyces Cerevisiae |
| massModifications | static: C+57.021464, variable: K+8.014199, R+10.008269, variable: S+79.966331 |
