| Metadata |
| datasetIdentifier | PASS00048 |
| datasetType | SRM |
| submitter | Aleksey Filimonov <filimonov.a.d@gmail.com> |
| submitter_organization | IMSB |
| lab_head_full_name | Sergei Moshkovskii |
| lab_head_email | sergei.moshkovskii@ibmh.msk.ru |
| lab_head_organization | IMSB |
| lab_head_country | Russia |
| datasetTag | Art_2012-02-23 |
| datasetTitle | Developing dMRM for Q6PI98, Q8N2U9, Q96DM3, Q9H3N8 |
| publicReleaseDate | 2012-04-20 00:00:00 |
| finalizedDate | 2012-03-20 16:13:39 |
| summary | Developing Dynamic MRM Method for Agilent MassHunter for proteins Q6PI98, Q8N2U9, Q96DM3, Q9H3N8 for researching proteome of 18th chromosome. Samples used: normal human plasma and human liver tissue. |
| contributors | Arthur T. Kopylov, Andrey Lisitsa, Sergei Moshkovskii |
| publication | unpublished |
| growth | |
| treatment | |
| extraction | |
| separation | Human liver tissue extracted proteins lysis in HFIP 20%; after reduction, alkylation and sigestion with trypsin, final concentration 1 ug/ul, original concentration 12.6 ug/ul.
Normal human plasma lysis in HFIP 20% after immunodepletion on Hu-14 column, reduction, alkylation and digestion with trypsin; the original concentration befor immunodepletion 53.17 mg/ml, after - 24.89 mg/ml (concentrated); in sample - 1 mg/ml. |
| digestion | |
| acquisition | |
| informatics | |
| instruments | Agilent Triple Quadrupole LC/MS 6490 |
| species | Human |
| massModifications | C+105.058 |
