| Metadata |
| datasetIdentifier | PASS00096 |
| datasetType | SRM |
| submitter | Olga Schubert <olga.schubert@gmail.com> |
| submitter_organization | IMSB |
| lab_head_full_name | Ruedi Aebersold |
| lab_head_email | aebersold@imsb.biol.ethz.ch |
| lab_head_organization | IMSB |
| lab_head_country | Switzerland |
| datasetTag | BCG_DosR_regulon |
| datasetTitle | Regulation of the DosR regulon under hypoxic stress in Mycobacterium bovis BCG |
| publicReleaseDate | 2013-05-14 00:00:00 |
| finalizedDate | 2013-05-15 01:13:04 |
| summary | DosR regulon |
| contributors | Olga Schubert |
| publication | Schubert, OT et al., The Mtb Proteome Library: A Resource of Assays to Quantify the Complete Proteome of Myco- bacterium tuberculosis, Cell Host & Microbe (2013), http://dx.doi.org/10.1016/j.chom.2013.04.008 |
| growth | Exponential phase cultures of Mycobacterium bovis BCG grown in Middlebrook 7H9 supplemented with 0.3% glycerol, 0.085% NaCl, 0.05% tyloxapol |
| treatment | Standing culture (shaking was stopped for 6 days) |
| extraction | Lysis buffer containing 8M Urea and 0.1% RapiGest. Bead-beating to break up the cells (3 x 10 min). |
| separation | |
| digestion | Trypsin |
| acquisition | SRM |
| informatics | Manual refinement using Skyline |
| instruments | Thermo Scientific TSQ Vantage |
| species | Mycobacterium bovis BCG |
| massModifications | static: C+57.021464, variable: K+8.014199, variable: R+10.008269 |
