| Metadata |
| datasetIdentifier | PASS00274 |
| datasetType | SRM |
| submitter | Nicole Plohnke <Nicole.Plohnke@rub.de> |
| submitter_organization | |
| lab_head_full_name | Sascha Rexroth |
| lab_head_email | Sascha.Rexroth@ruhr-uni-bochum.de |
| lab_head_organization | Ruhr University Bochum |
| lab_head_country | Germany |
| datasetTag | NP01SRM |
| datasetTitle | SRM-analysis of Podospora anserina mitochondria |
| publicReleaseDate | 2014-03-15 00:00:00 |
| finalizedDate | 2014-03-05 02:53:28 |
| summary | Here we report data of an analysis to monitor the age-related quantitative protein composition of the mitochondria of the fungal aging model Podospora anserina. For a more detailed analysis, a targeted proteome analysis was applied focusing on 15 proteins from respiratory, ROS-scavenging and quality control pathways. Analyzing six distinct age-stages from juvenile to senescent P. anserina cultures revealed low, but statistically significant changes for the mitochondrial respiratory complexes.
A P. anserina PaSod3 over-expression mutant with a phenotype of mitochondrial ROS over-production was used for biological evaluation of changes observed during aging. LC-MS analysis of the mutant revealed severe changes to the mitochondrial proteome – substantially larger than observed during senescence. The vast difference between aging in the wild type and ROS stress in the mutant suggest that oxidative stress within the mitochondria is not the dominating mechanism for the aging process in P. anserina.
|
| contributors | Plohnke, Nicole
Rexroth, Sascha |
| publication | Plohnke, N, Hamann, A, Poetsch, A, Osiewacz, HD, Rögner M and Rexroth S, Proteomic analysis of mitochondria from senescent Podospora anserina casts new light on ROS dependent aging mechanisms, Experimental Gerontology, in Press |
| growth | |
| treatment | |
| extraction | |
| separation | |
| digestion | |
| acquisition | |
| informatics | |
| instruments | Thermo Scientific TSQ Vantage |
| species | Podospora anserina |
| massModifications | K+8.014199, R+10.008269 |
