| Metadata |
| datasetIdentifier | PASS00397 |
| datasetType | SRM |
| submitter | Martin Soste <martin.soste@bc.biol.ethz.ch> |
| submitter_organization | ETH Zurich |
| lab_head_full_name | Paola Picotti |
| lab_head_email | paola.picotti@bc.biol.ethz.ch |
| lab_head_organization | ETH Zurich |
| lab_head_country | Switzerland |
| datasetTag | senti_SRM_quant |
| datasetTitle | SRM label-free quantification of sentinel proteins, phosphopeptides and degradation products across 8 perturbations of yeast and their corresponding controls. |
| publicReleaseDate | 2014-09-07 00:00:00 |
| finalizedDate | 2014-01-23 15:27:06 |
| summary | YKL103C (APE1) was quantified from the raw files containing "ape1" in the filename. RPS6 (RAS[167]S[167]LKA) in the osmo-stress, rapamycin, AA/N starvation and stationary phase experiments was quantified in the raw files containing "rps6..." in the filename because the window for scheduling was wrong in the original analysis of all sentinels. The same applies for quantification of CKI1 (RHS[167]LTR) and YAK1 (RAS[167]LNSK) in the heat shock experiment. The quantification for these phosphopeptides is also from the raw file containing "cki1_yak1..." in the filename. |
| contributors | Soste, M, Hrabakova, R, Wanka, S, Melnik, A, Boersema, P, Maiolica, A, Wernas, T, Tognetti, M, von Mering, C, Picotti, P. |
| publication | Soste, M, Hrabakova, R, Wanka, S, Melnik, A, Boersema, P, Maiolica, A, Wernas, T, Tognetti, M, von Mering, C, and Picotti, P, A sentinel protein assay for the simultaneous, large-scale quantification of cellular process activities, Nat. Methods, submitted |
| growth | |
| treatment | |
| extraction | |
| separation | |
| digestion | |
| acquisition | |
| informatics | |
| instruments | AB Sciex QTRAP 5500 |
| species | Saccharomyces cerevisiae |
| massModifications | static: C+57.021465 variable: STY+79.966331 |
