| Metadata |
| datasetIdentifier | PASS00401 |
| datasetType | SRM |
| submitter | Paolo Cifani <paolo.cifani@immun.lth.se> |
| submitter_organization | Immunotechnology dept., Lund University, Sweden |
| lab_head_full_name | Peter James |
| lab_head_email | peter.james@immun.lth.se |
| lab_head_organization | Immunotechnology dept., Lund University, Sweden |
| lab_head_country | Sweden |
| datasetTag | IrradiationDNArep |
| datasetTitle | DNA-repair enzymes measure before and after gamma-irradiation of breast cancer-derived cell lines |
| publicReleaseDate | 2014-06-01 00:00:00 |
| finalizedDate | 2014-01-24 03:22:28 |
| summary | Targeted measurement of DNA-repair enzymes upon treatment of cells with gamma-radiation. Samples were collected in 5 independent biological replicates per condition. |
| contributors | paolo.cifani@immun.lth.se |
| publication | unpublished |
| growth | Cells were cultivated in RPMI1640 + 10%FBS and L-glutammine, in standard condition. |
| treatment | Cells were irradiated using a 137Cs source. 60 minutes after treatment, cells were washed, detached with trypsin, pelletted and frozen. |
| extraction | Cell pellets were resuspended in Laemmli buffer, boiled for 5 minutes and loaded on a SDS-PAGE gel. One single slab was excised and proteins were in-gel reduced, alkilated and trypsin digested. |
| separation | |
| digestion | One single slab was excised and proteins were in-gel reduced, alkilated and trypsin digested. |
| acquisition | Peptides were analysed by SRM using a Thermo TSQ Vantage. |
| informatics | Skyline was used to both generate the transition list and analyse the raw MS-files. |
| instruments | Thermo Scientific Vantage TSQ |
| species | Human |
| massModifications | C+57.021464. |
