| Metadata |
| datasetIdentifier | PASS00716 |
| datasetType | SRM |
| submitter | Thierry Schmidlin <t.t.schmidlin@uu.nl> |
| submitter_organization | Utrecht University |
| lab_head_full_name | Albert J. R. Heck |
| lab_head_email | a.j.r.heck@uu.nl |
| lab_head_organization | Utrecht University |
| lab_head_country | Netherlands |
| datasetTag | neuropeptideSRM |
| datasetTitle | Quantifying diet-induced expression of highly charged endogenous neuropeptides in rat hypothalami |
| publicReleaseDate | 2015-07-08 00:00:00 |
| finalizedDate | 2019-01-23 02:42:06 |
| summary | |
| contributors | Thierry Schmidlin, Arjen J. Boender, Christian K. Frese, Albert J. R. Heck, Roger A. H. Adan, A. F. Maarten Altelaar |
| publication | PMID: 26376940 |
| growth | |
| treatment | rats subjected to three different diets for 8 days:
CTRL: ad libitum access to water and chow
HFHS: ad libitum access to water, chow and saturated fat
RFS: ad libitum access to water, access to chow restricted to two hours per day |
| extraction | neuropeptide extracted from hypothalami by double extraction (acidified acetone, diluted acetic acid) |
| separation | separation occured on a 25 cm, 75 µm ID PepMap RSLC, C18, 100 Å, 2 µm particle size column (Thermo Scientific, Odense, DK)
Gradient: 5-20%B in 70 minutes, 20-40%B in 10 minutes, 40-100%B in 2 minutes, 100%B for 8 minutes
Flow rate: 200 nl/min |
| digestion | none |
| acquisition | scheduled SRM experiments on a TSQ Vantage, 8 minutes time window, Q1/Q3 at resolution of 0.7 m/z half maximum peak width, cycle time of 2 seconds |
| informatics | AUC quantification using Skyline
SRMStats for statistical analysis |
| instruments | TSQ Vantage |
| species | Rats |
| massModifications | static: C+57.021464, variable: C-term(amidated)-.984016, Gln->pyro-Gln(N-term Q)-17.026549 |
