| Metadata |
| datasetIdentifier | PASS00053 |
| datasetType | XlinkMS |
| submitter | Lars Malmstroem <lars@imsb.biol.ethz.ch> |
| submitter_organization | |
| lab_head_full_name | |
| lab_head_email | |
| lab_head_organization | |
| lab_head_country | |
| datasetTag | xlms_on_pp2a |
| datasetTitle | Structural Probing of a Protein Phosphatase 2A Network by Chemical Cross-linking and Mass Spectrometry |
| publicReleaseDate | 2012-07-26 00:00:00 |
| finalizedDate | 2012-07-26 01:33:28 |
| summary | Mass spectrometry is routinely applied to the identification and quantification of proteins and
post-translational modifications. By combining chemical cross-linking and mass spectrometry
(XL-MS), we gained structural information in the form of distance restraints on an interaction
network of protein complexes isolated from human cells. Systematic XL-MS analysis of
protein phosphatase 2A (PP2A) complexes identified 176 inter-protein and 569 intra-protein
spatial restraints that link specific trimeric PP2A complexes to a multitude of adaptor proteins
controlling its cellular function. Cross-links guided molecular modeling of the binding
interface between immunoglobulin-binding-protein-1 (IGBP1) and PP2A and revealed the
topology of a TCP-1-ring-complex (TRiC) chaperonin interacting with the PP2A regulatory
subunit Bγ. This study establishes XL-MS as an integral part of hybrid structural biology
approaches for the analysis of endogenous protein complexes.
|
| contributors | Franz Herzog
Lars Malmstroem |
| publication | Structural Probing of a Protein Phosphatase 2A Network by Chemical
Cross-linking and Mass Spectrometry
Franz Herzog Abdullah Kahraman Daniel Böhringer Raymond Mak Andreas
Bracher Thomas Walzthöni Alexander Leitner Martin Beck Franz-Ulrich Hartl Nenad
Ban Lars Malmström and Ruedi Aebersold
submitted |
| growth | |
| treatment | |
| extraction | |
| separation | |
| digestion | |
| acquisition | |
| informatics | |
| instruments | LTQ Orbitrap |
| species | Human |
| massModifications | static: C+57.021464
variable: K+156.0786442
variable: K+155.0964278 |
