| Metadata |
| datasetIdentifier | PASS00503 |
| datasetType | SRM |
| submitter | Nicole Plohnke <Nicole.Plohnke@rub.de> |
| submitter_organization | Ruhr-Universität Bochum |
| lab_head_full_name | Sascha Rexroth |
| lab_head_email | Sascha.Rexroth@rub.de |
| lab_head_organization | Plant biochemistry |
| lab_head_country | Germany |
| datasetTag | NP_Syn_LAHG_01 |
| datasetTitle | SRM lipid analysis of Synechocystis sp. PCC 6803 cultivated under mixotrophic conditions and light-activated heterotrophic growth |
| publicReleaseDate | 2014-08-30 00:00:00 |
| finalizedDate | 2014-05-21 07:11:22 |
| summary | |
| contributors | Nicole Plohnke
Sascha Rexroth |
| publication | Plohnke, N, Seidel, T, Kahmann, U, Rögner, M, Schneider, D, and Rexroth, S, The proteome and lipidome of Synechocystis sp. PCC 6803 cells grown under light-activated heterotrophic conditions, unpublished |
| growth | |
| treatment | |
| extraction | Lipids were extracted using chloroform:methanol according to the method of Bligh and Dyer (Bligh, E. G., and Dyer, W. J. (1959) A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37, 911-917) |
| separation | |
| digestion | |
| acquisition | Lipid analysis was carried out by ESI-MS/MS after direct infusion with a flow rate of 5 µL/min into a triple quadrupole mass spectrometer (TSQ Vantage, Thermo Scientific). Lipids were detected by selected reaction monitoring (SRM) using the settings adapted from (Welti, R., Wang, X., and Williams, T. D. (2003) Electrospray ionization tandem mass spectrometry scan modes for plant chloroplast lipids. Anal. Biochem. 314, 149-152). MGDG and DGDG were analyzed in positive mode. The ion spray voltage was set to 3500 V and 5 sheath gas was added. SQDG and PG were analyzed in negative mode and the ion spray voltage was set to 3000 V and 20 sheath gas was added. During all acquisitions, the temperature of the ion transferring tube was set to 270 °C and collision-activated dissociation pressure was set to 1.5 mTorr. |
| informatics | |
| instruments | Thermo Scientific TSQ Vantage |
| species | Synechocystis sp. PCC 6803 |
| massModifications | none |
