| Metadata |
| datasetIdentifier | PASS00753 |
| datasetType | MSMS |
| submitter | Mike Buckley <m.buckley@manchester.ac.uk> |
| submitter_organization | The University of Manchester |
| lab_head_full_name | Mike Buckley |
| lab_head_email | m.buckley@manchester.ac.uk |
| lab_head_organization | The University of Manchester |
| lab_head_country | United Kingdom |
| datasetTag | Giant_Sloth_Bone |
| datasetTitle | Bone proteomes of extinct giant ground sloths Megatherium and Lestodon compared with extant two-toed sloth (Choloepus) and three-toed sloth (Bradypus) bone proteomes. |
| publicReleaseDate | 2015-10-01 00:00:00 |
| finalizedDate | 2015-10-01 07:52:15 |
| summary | Bone proteome extracts, reduced, alkylated and digested with trypsin. |
| contributors | M. Buckley |
| publication | Buckley, M. Fariņa, RA. Lawless C., Tambusso, PS. Varela, L. Carlini, AA. Powell, JE. Martinez, JG. Collagen sequence analysis of the extinct giant ground sloths Lestodon and Megatherium. PlosONE submitted. |
| growth | Not applicable |
| treatment | None |
| extraction | Demineralisation of bone mineral with 0.6 M hydrochloric acid. Solubilisation of acid insoluble pellet using guanidine hydrochloride. Ultrafiltration into ammonium bicarbonate using 10 kDa filter. Reduction and alkylation (with iodoacetamide). |
| separation | HPLC |
| digestion | Trypsin |
| acquisition | Peptides were then automatically selected for fragmentation by data-dependent analysis; 6 MS/MS scans (Velos ion trap, product ion scans, rapid scan rate, Centroid data; scan event: 500 count minimum signal threshold, top 6) were acquired per cycle, dynamic exclusion was employed, and 1 repeat scan (2 MS/MS scans total) was acquired in a 30 s repeat duration with that precursor being excluded for the subsequent 30 s (activation: CID, 2+ default charge state, 2 m/z isolation width, 35 eV normalised collision energy, 0.25 Activation Q, 10.0 ms Activation time). |
| informatics | Not applicable |
| instruments | Thermo Scientific LTQ Orbitrap Elite |
| species | Sloth |
| massModifications | C+57.021464 |
