| Metadata |
| datasetIdentifier | PASS00861 |
| datasetType | MSMS |
| submitter | Marco Trevisan-Herraz <mtrevisan@cnic.es> |
| submitter_organization | Centro Nacional de Investigaciones Cardiovasculares (CNIC) |
| lab_head_full_name | Jesús Vázquez |
| lab_head_email | jvazquez@cnic.es |
| lab_head_organization | Centro Nacional de Investigaciones Cardiovasculares (CNIC) |
| lab_head_country | Spain |
| datasetTag | HDL-AAA |
| datasetTitle | Quantitative proteomics analysis of high density lipoproteins (HDL) in human abdominal aortic aneurysm (AAA) |
| publicReleaseDate | 2016-09-01 00:00:00 |
| finalizedDate | |
| summary | Proteomics analysis in HDL particle isolated from 14 AAA and 7 control subjects. A total of 7 independent iTRAQ 4-plex experiments were performed. HDL particles were isolated from plasma by ultracentrifugation and subjected to digestion. The resulting peptides were iTRAQ-labeled and were subjected to high-throughput LC-MS/MS analysis. |
| contributors | Inmaculada Jorge, Emilio Camafeita, Marco Trevisan-Herraz |
| publication | Elena Burillo, Inmaculada Jorge, Diego Martínez-López, Emilio Camafeita, Luis Miguel Blanco-Colio, Marco Trevisan-Herraz, Jesús Egido, Jean-Baptiste Michel, Olivier Meilhac, Jesús Vázquez and Jose Luis Martin-Ventura, submitted |
| growth | N/A |
| treatment | N/A |
| extraction | Lipoproteins were isolated from individual EDTA plasma of AAA patients and controls by ultracentrifugation. |
| separation | N/A |
| digestion | HDL samples were in-gel digested overnight at 37oC with sequencing grade trypsin. |
| acquisition | The resulting tryptic peptide mixtures were subjected to nano-LC-MS. High-resolution analyses was performed using a nano-HPLC Easy nLC 1,000 (Thermo Scientific, San Jose, CA, USA) coupled to a QExactive mass spectrometer (Thermo Scientific). |
| informatics | Peptide identification was carried out by searching against a Human Uniprot database using the SEQUEST algorithm (Proteome Discoverer 1.4, Thermo Finnigan). |
| instruments | Thermo Scientific Q Exactive |
| species | Human |
| massModifications | static: C+57.021
variable: M+15.995, K+144.102, N-terminus+144.102 |
