| Metadata |
| datasetIdentifier | PASS01290 |
| datasetType | SRM |
| submitter | Dingyin Tao <dingyin.tao@nih.gov> |
| submitter_organization | W. Harry Feinstone Department of Molecular Microbiology & Immunology, Johns Hopkins Bloomberg School of Public Health |
| lab_head_full_name | Rhoel R. Dinglasan |
| lab_head_email | rdinglasan@epi.ufl.edu |
| lab_head_organization | EMERGING PATHOGENS INSTITUTE, University of Florida |
| lab_head_country | United States |
| datasetTag | PSSP17 |
| datasetTitle | 364 MRM RAW data for paper: A saliva-based rapid test to quantify the infectious subclinical malaria parasite reservoir |
| publicReleaseDate | 2018-11-15 00:00:00 |
| finalizedDate | 2018-11-13 08:44:38 |
| summary | A large proportion of ongoing malaria parasite transmission is attributed to low density, subclinical infections not readily detected by available rapid diagnostic tests (RDTs) or microscopy. Plasmodium falciparum gametocyte carriage is subclinical but gametocytemic individuals comprise the parasite reservoir that leads to infection of mosquitoes and local transmission. Effective detection and quantification of these carriers can help advance malaria elimination strategies. However, no point- of -need (PON) RDTs for gametocyte detection exist, much less one that is based on thecan perform non-invasive sampling of saliva outside a clinical setting. Here, we report on the discovery of 35 parasite biomarkers and thefrom which we down-selection selected to a single candidate for use in a PON RDT. We performed a cross-sectional, multi-OMICs study of saliva from 364 subclinical children with subclinical infection in Cameroon and Zambia and produced a prototype saliva-based PON lateral flow-immunoassay test for P. falciparum gametocyte carriers. The test that is capable of identifying submicroscopic carriage in both clinical and non-clinical settings, and is effective compatible with archived saliva samples. |
| contributors | Dingyin Tao and Rhoel R. Dinglasan |
| publication | unpublished |
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| informatics | |
| instruments | Agilent 6490 QQQ |
| species | Human, Plasmodium falciparum |
| massModifications | None |
