| summary | High capacity streptavidin agarose (Thermo Scientific) was incubated with C-terminal
biotin-tagged 20 amino acid N-terminal peptides of H4, H4R3me2a, and H4R3me2s
peptide immunoprecipitates from nuclear extracts of HCT116 cells, separated by
sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), and
stained with SimplyBlue Safestain (Invitrogen). Protein bands of interest were excised
and subjected to electrospray–ion trap tandem mass spectrometry (LCQ-Deca,
Finnigan) |
| publication | Bing Yao1, †, Tao Gui1, †, Xiangwei Zeng1, Yexuan Deng1, Zhi Wang1, Ying Wang1,Dongjun Yang1, Qixiang Li1, Peipei Xu1, Ruifeng Hu1, Xinyu Li1, Bing Chen1,Jing Wang1, Ke Zen1, Haitao Li2, Melissa J. Davis3, Marco J. Herold3, Hua-Feng Pan4,Zhi-Wei Jiang4, David C. S. Huang3, Ming Liu1*, Junyi Ju1*and Quan Zhao1*.Genome Medicine.submitted. |
