| Metadata |
| datasetIdentifier | PASS01660 |
| datasetType | SRM |
| submitter | Koen De Cremer <Koen.DeCremer@sciensano.be> |
| submitter_organization | Sciensano |
| lab_head_full_name | Koen De Cremer |
| lab_head_email | Koen.decremer@sciensano.be |
| lab_head_organization | Sciensano |
| lab_head_country | Belgium |
| datasetTag | MRMurinerespikid |
| datasetTitle | MRM method for urinary proteins related to respiratory health or for physiological adjustment |
| publicReleaseDate | 2021-04-03 00:00:00 |
| finalizedDate | |
| summary | development and application of an MRM method to urinary samples of children for the proteins CC16 (P11684), B2M (P61769), OPN (P10451), RBP4 (P02753), HSA (P02768), MYO (P02144), NFKB (Q04206) and BSA (P02769) |
| contributors | Sarah Nauwelaerts, Nancy Roosens, Alfred Bernard, Sigrid De Keersmaecker and Koen De Cremer |
| publication | Nauwelaerts et al., Scientific Reports, Development of a multiplex mass spectrometry method for simultaneous quantification of urinary proteins related to respiratory health, submitted
|
| growth | |
| treatment | |
| extraction | Proteins were extracted from the urine using a methanol precipitation at -20C |
| separation | Peptides were separated on a Acquity UPLC BEH C18 1.7 um, 2.1 x 100 mm column with solvent A water + 0.1% FA and solvent B acetonitrile + 0.1% FA |
| digestion | a standard overnight trypsin was used |
| acquisition | MRM were optimized for the different peptides/proteins (see excel sheet) |
| informatics | raw data were analyzed using the Targetlynx software from Waters |
| instruments | Waters Xevo TQ-S |
| species | Human |
| massModifications | K+8.014199, R+10.008269 |
