| Metadata |
| datasetIdentifier | PASS03809 |
| datasetType | SRM |
| submitter | Laurine Pigosso <laurinepigosso@gmail.com> |
| submitter_organization | Universidade Federal de Goiás |
| lab_head_full_name | Laboratorio de Biologia Molecular |
| lab_head_email | cmasoares@gmail.com |
| lab_head_organization | Goias |
| lab_head_country | Brazil |
| datasetTag | proteomic |
| datasetTitle | The response of Paracoccidioides lutzii with human neutrophils |
| publicReleaseDate | 2023-02-08 00:00:00 |
| finalizedDate | 2023-02-15 18:24:19 |
| summary | |
| contributors | Lilian Cristiane Baeza
Laurine Lacerda Pigosso
Maristela Pereira
Célia Maria de Almeida Soares |
| publication | unpublished |
| growth | |
| treatment | |
| extraction | |
| separation | |
| digestion | |
| acquisition | |
| informatics | |
| instruments | Chromatography was performed on a precolumn nanoEaseTM 5 mm x BridgeTM BEH130 C18 300 mm x 50 mm; trap column 5 mm, 180 mm x 20 mm and analytical reversed-phase column BEH130 C18 1.7 mm, 100 mm x 100 mm (Waters Corp., Milford, MA, USA) using an nanoACQUITY UPLCTM system (Waters Corp., Milford, MA, USA). |
| species | Paracoccidioides lutzii |
| massModifications | strict trypsin specificity, allowing up one missed cleavages; minimum peptide length was five amino acids; the detection of at least two fragment ions per peptide; carbamidomethylation of cysteine was a fixed modification; maximum protein mass (600 kDa); and phosphoryl STY of proteins and oxidation of methionine were set as variable modifications. |
